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AB235827

Anti-ACAP1 antibody

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(1 Publication)

Rabbit Polyclonal ACAP1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACAP1.

View Alternative Names

CENTB1, KIAA0050, ACAP1, Centaurin-beta-1, Cnt-b1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAP1 antibody (AB235827)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAP1 antibody (AB235827)

Paraffin-embedded human pancreas tissue stained for ACAP1 using ab235827 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAP1 antibody (AB235827)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAP1 antibody (AB235827)

Paraffin-embedded human lung tissue stained for ACAP1 using ab235827 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ACAP1 antibody (AB235827)
  • WB

Supplier Data

Western blot - Anti-ACAP1 antibody (AB235827)

All lanes:

Western blot - Anti-ACAP1 antibody (ab235827) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACAP1.

Q15027

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAP1 also known as ADP-ribosylation factor GTPase-activating protein 1 weighs around 81 kDa. This protein functions as a GTPase-activating protein and plays a significant role in intracellular trafficking and cargo sorting. ACAP1 is expressed in various tissues including lungs and kidneys and typically resides in the cytoplasm and associated vesicular membranes. Its precise role in cellular transport mechanisms lies in its ability to regulate the activity of the ARF family of small GTPases which are pivotal to vesicle budding and trafficking.
Biological function summary

ACAP1 engages in both cellular membrane dynamics and actin cytoskeleton organization. It forms part of a multiprotein complex interacting with molecules like clathrin and cargo adaptors to mediate endocytosis and recycling pathways. In addition to its involvement in vesicular transport ACAP1 participates in actin-driven changes in membrane structure necessary for effective cargo handling and cellular response to extracellular signals. This dual functionality emphasizes its important role in maintaining cellular homeostasis.

Pathways

ACAP1 involvement extends to the Arf6 signaling pathway and the endocytic recycling pathway. These pathways highlight its function in cellular internalization and receptor recycling. ACAP1 interacts with proteins such as GRK2 and AP-2 in these pathways facilitating the orchestrated regulation of cargo uptake and recycling at the plasma membrane. Moreover its actions influence intracellular signal transduction processes showcasing its importance in maintaining cellular communication and environmental interaction.

ACAP1 associates with conditions such as cancer and type 2 diabetes. In cancer dysregulation of ACAP1 can lead to aberrant cellular growth and migration due to its role in cell surface receptor recycling and turnover. Abnormalities in ACAP1 function also relate to diabetes as impaired endocytosis and insulin receptor recycling can affect glucose homeostasis. Further connection to these diseases occurs via interactions with proteins like Rab11 and Eps15 indicating that ACAP1 impacts pathophysiological pathways and represents a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
See full target information ACAP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35625704

2022

Arsenic Activates the ER Stress-Associated Unfolded Protein Response via the Activating Transcription Factor 6 in Human Bronchial Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Wadgaonkar,Zhuoyue Bi,Junmei Wan,Yao Fu,Qian Zhang,Bandar Almutairy,Wenxuan Zhang,Yiran Qiu,Chitra Thakur,Maik Hüttemann,Fei Chen
View all publications
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Product promise

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