AB191424

Anti-ACAP2 antibody [EPR15721]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ACAP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

CENTB2, KIAA0041, ACAP2, Centaurin-beta-2, Cnt-b2

5 Images

Supplier Data

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

All lanes:

Western blot - Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 88 kDa

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Supplier Data

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

All lanes:

Western blot - Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

Lane 1:

293 lysate at 10 µg

Lane 2:

MOLT4 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

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Supplier Data

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

All lanes:

Western blot - Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/5000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

false

Flow Cytometry (Intracellular) - Anti-ACAP2 antibody [EPR15721] (AB191424)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ACAP2 antibody [EPR15721] (AB191424)

Intracellular flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells (paraformaldehyde-fixed, 2%)labeling ACAP2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

All lanes:

Western blot - Anti-ACAP2 antibody [EPR15721] (ab191424) at 1/1000 dilution

Lane 1:

RAW 264.7 lysate at 10 µg

Lane 2:

PC12 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 88 kDa

false

Carrier free

Anti-ACAP2 antibody [EPR15721] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15721

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/270", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAP2 also known as ArfGAP with coiled-coil ankyrin repeat and PH domain-containing protein 2 plays a role in cell signaling and membrane trafficking. It has a molecular mass of around 90 kDa and is expressed in various tissues including the brain and kidney. ACAP2 acts as a GTPase-activating protein (GAP) for ADP-ribosylation factor 6 (Arf6) a member of the Arf family which is important in vesicle formation in cellular processes.

Biological function summary

ACAP2 influences cellular processes by regulating actin cytoskeleton remodeling and endocytosis. It is part of a complex that interacts with proteins like phosphatidylinositol 45-bisphosphate which helps localize ACAP2 to specific cellular membranes. The protein's ability to bind and hydrolyze GTP enables changes in cell shape and movement contributing to cellular migration and morphological adjustments.

Pathways

ACAP2 participates in the endocytic pathway which is important for internalizing substances into the cell. It is also involved in the signaling pathway related to the actin cytoskeleton impacting cell motility. In these pathways ACAP2 interacts with proteins such as Arf6 facilitating its function as a regulator of endocytosis and actin dynamics.

ACAP2 has been linked to cancer and neurodegenerative diseases. Dysregulation of ACAP2 and its associated proteins like Arf6 can lead to changes in cell adhesion and motility processes often altered in tumor progression. Additionally aberrant ACAP2 function can interfere with normal neurological processes possibly contributing to the pathology of neurodegenerative disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).

See full target information ACAP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 15:1018-1035 PubMed37065546

2023

ADCY9 functions as a novel cancer suppressor gene in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yijie Tang,Tianyi Wang,Anping Zhang,Jiaqi Zhu,Tingting Zhou,You-Lang Zhou,Jiahai Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAP2 antibody [EPR15721]

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

Flow Cytometry (Intracellular) - Anti-ACAP2 antibody [EPR15721] (AB191424)

Western blot - Anti-ACAP2 antibody [EPR15721] (AB191424)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise