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AB232615

Anti-ACAP2 antibody [EPR15721] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ACAP2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

CENTB2, KIAA0041, ACAP2, Centaurin-beta-2, Cnt-b2

1 Images
Flow Cytometry (Intracellular) - Anti-ACAP2 antibody [EPR15721] - BSA and Azide free (AB232615)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ACAP2 antibody [EPR15721] - BSA and Azide free (AB232615)

Intracellular flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells (paraformaldehyde-fixed, 2%)labeling ACAP2 with ab191424 at 1/270 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191424).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15721

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab232615 is the carrier-free version of ab191424.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAP2 also known as ArfGAP with coiled-coil ankyrin repeat and PH domain-containing protein 2 plays a role in cell signaling and membrane trafficking. It has a molecular mass of around 90 kDa and is expressed in various tissues including the brain and kidney. ACAP2 acts as a GTPase-activating protein (GAP) for ADP-ribosylation factor 6 (Arf6) a member of the Arf family which is important in vesicle formation in cellular processes.
Biological function summary

ACAP2 influences cellular processes by regulating actin cytoskeleton remodeling and endocytosis. It is part of a complex that interacts with proteins like phosphatidylinositol 45-bisphosphate which helps localize ACAP2 to specific cellular membranes. The protein's ability to bind and hydrolyze GTP enables changes in cell shape and movement contributing to cellular migration and morphological adjustments.

Pathways

ACAP2 participates in the endocytic pathway which is important for internalizing substances into the cell. It is also involved in the signaling pathway related to the actin cytoskeleton impacting cell motility. In these pathways ACAP2 interacts with proteins such as Arf6 facilitating its function as a regulator of endocytosis and actin dynamics.

ACAP2 has been linked to cancer and neurodegenerative diseases. Dysregulation of ACAP2 and its associated proteins like Arf6 can lead to changes in cell adhesion and motility processes often altered in tumor progression. Additionally aberrant ACAP2 function can interfere with normal neurological processes possibly contributing to the pathology of neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6). Doesn't show GAP activity for RAB35 (PubMed : 30905672).
See full target information ACAP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:103 PubMed40515797

2025

GelMA hydrogel-loaded extracellular vesicles derived from keratinocytes promote skin microvasculature regeneration and wound healing in diabetic mice through activation of the PDGF-induced PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Leilei Zhou,Wenqiang Li,Weiheng Zhao,Weimin Chen,Mohammed S AlQranei,Jiarui Bi,Ping Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com