AB154396

Anti-ACAT1 antibody

Name: Anti-ACAT1 antibody (ab154396) | Abcam
Brand: Abcam Limited
SKU: AB154396
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal ACAT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACAT1 aa 1-200.

View Alternative Names

ACAT, MAT, ACAT1, Acetoacetyl-CoA thiolase, T2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (AB154396)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (AB154396)

Paraffin embedded human normal kidney tissue stained for ACAT1 using ab154396 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (AB154396)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (AB154396)

A431 cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (AB154396)

HeLa cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.

Lab

Western blot - Anti-ACAT1 antibody (AB154396)

Lanes 1 - 4 : Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab154396 was shown to react with ACAT1 in western blot. The band observed in the knockout cell line ab269621 (knockout cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ACAT1 antibody (ab154396) at 1/500 dilution

Lane 1:

Wild-type SW480 cell lysate at 20 µg

Lane 2:

ACAT1 CRISPR/Cas9 edited SW480 cell lysate at 20 µg

Lane 2:

Western blot - Human ACAT1 knockout SW480 cell line (<a href='/en-us/products/cell-lines/human-acat1-knockout-sw480-cell-line-ab269621'>ab269621</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Lab

Western blot - Anti-ACAT1 antibody (AB154396)

Lanes 1 - 4 : Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab154396 was shown to react with ACAT1 in western blot. The band observed in knockout cell line ab269621 (knockout cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ACAT1 antibody (ab154396) at 1/500 dilution

Lane 1:

Wild-type SW480 cell lysate at 20 µg

Lane 2:

ACAT1 knockout SW480 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Unknown

Western blot - Anti-ACAT1 antibody (AB154396)

10% SDS PAGE

All lanes:

Western blot - Anti-ACAT1 antibody (ab154396) at 1/500 dilution

Lane 1:

HEK-293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 45 kDa

false

Unknown

Western blot - Anti-ACAT1 antibody (AB154396)

10% SDS PAGE

All lanes:

Western blot - Anti-ACAT1 antibody (ab154396) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-ACAT1 antibody (AB154396)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ACAT1 antibody (ab154396) at 1/1000 dilution

All lanes:

whole zebrafish lysate at 30 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Zebrafish

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACAT1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P24752

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAT1 also known as acyl-CoA:cholesterol acyltransferase 1 is an enzyme that catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty acyl-CoA. It has a molecular mass of approximately 50 kDa. ACAT1 is present in the endoplasmic reticulum membrane and is expressed widely including in liver adrenal glands and macrophages. The enzyme's activity is significant in lipid metabolism storing cholesterol as cholesteryl esters.

Biological function summary

ACAT1 plays a central role in cellular cholesterol homeostasis and in preventing cholesterol-induced cytotoxicity by converting free cholesterol into inert cholesteryl esters for storage. It functions as a homotetramer suggesting that it works as a part of a larger protein complex. ACAT1 influences lipid accumulation within cells and mediates the regulation of cholesterol levels which is vital for maintaining membrane integrity and cellular function.

Pathways

ACAT1 participates in the cholesterol metabolism pathway and the lipid biosynthesis pathway. It interacts with proteins such as LDLR (low-density lipoprotein receptor) by controlling the availability of free cholesterol within cells. These pathways are critical in maintaining lipid balance and influencing lipoprotein metabolism. ACAT1's enzymatic activity impacts how cells manage cholesterol influx and storage linking to broader lipid regulation networks.

ACAT1 is associated with atherosclerosis and Alzheimer's disease. The conversion of cholesterol into cholesteryl esters affects the development of atherosclerotic plaques contributing to cardiovascular disease risk. In Alzheimer's disease ACAT1 may influence the accumulation of amyloid-beta a protein linked to the pathology of the disease. The protein APP (amyloid precursor protein) connects to ACAT1 as disturbances in cholesterol metabolism can affect amyloid-beta production linking this enzyme to neurodegenerative conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed : 1715688, PubMed : 7728148, PubMed : 9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed : 1715688, PubMed : 7728148, PubMed : 9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed : 17371050). Thereby, it plays a major role in ketone body metabolism (PubMed : 1715688, PubMed : 17371050, PubMed : 7728148, PubMed : 9744475).

See full target information ACAT1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38534399

2024

Modulating Cholesterol Metabolism via ACAT1 Knockdown Enhances Anti-B-Cell Lymphoma Activities of CD19-Specific Chimeric Antigen Receptor T Cells by Improving the Cell Activation and Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Su,Jie Yao,Muhammad Asad Farooq,Iqra Ajmal,Yixin Duan,Cong He,Xuefei Hu,Wenzheng Jiang

BMC oral health 22:126 PubMed35428233

2022

Identification and validation of transcription factor-driven enhancers of genes related to lipid metabolism in metastatic oral squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Liru Zhang,Shuangling Zhao,Yuanhang Liu,Feifei Lv,Xixue Geng

Lipids in health and disease 21:22 PubMed35172832

2022

Correlation of cholesteryl ester metabolism to pathogenesis, progression and disparities in colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirong Liu,Christian R Gomez,Ingrid Espinoza,Thuy Phuong T Le,Veena Shenoy,Xinchun Zhou

PPAR research 2016:7631085 PubMed27057154

2016

Inducible Conditional Vascular-Specific Overexpression of Peroxisome Proliferator-Activated Receptor Beta/Delta Leads to Rapid Cardiac Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Kay-Dietrich Wagner,Ana Vukolic,Delphine Baudouy,Jean-François Michiels,Nicole Wagner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (AB154396)

Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (AB154396)

Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (AB154396)

Western blot - Anti-ACAT1 antibody (AB154396)

Western blot - Anti-ACAT1 antibody (AB154396)

Western blot - Anti-ACAT1 antibody (AB154396)

Western blot - Anti-ACAT1 antibody (AB154396)

Western blot - Anti-ACAT1 antibody (AB154396)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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