Name: Anti-ACAT1 antibody [EPR10359]
SKU: ab168342
Rating: 5 (3 reviews)

Rabbit Recombinant Monoclonal ACAT1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 18 publications.

Images

Western blot - Anti-ACAT1 antibody [EPR10359] (AB168342), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Not recommended	Tested	Not recommended
Mouse	Predicted	Predicted	Not recommended	Predicted	Not recommended
Rat	Predicted	Predicted	Not recommended	Predicted	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information ACAT1

Function

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:1715688, PubMed:17371050, PubMed:7728148, PubMed:9744475).

Alternative names

ACAT, MAT, ACAT1, Acetoacetyl-CoA thiolase, T2

Recommended products

Rabbit Recombinant Monoclonal ACAT1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 18 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10359
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ACAT1 also known as acyl-CoA:cholesterol acyltransferase 1 is an enzyme that catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty acyl-CoA. It has a molecular mass of approximately 50 kDa. ACAT1 is present in the endoplasmic reticulum membrane and is expressed widely including in liver adrenal glands and macrophages. The enzyme's activity is significant in lipid metabolism storing cholesterol as cholesteryl esters.

Biological function summary

ACAT1 plays a central role in cellular cholesterol homeostasis and in preventing cholesterol-induced cytotoxicity by converting free cholesterol into inert cholesteryl esters for storage. It functions as a homotetramer suggesting that it works as a part of a larger protein complex. ACAT1 influences lipid accumulation within cells and mediates the regulation of cholesterol levels which is vital for maintaining membrane integrity and cellular function.

Pathways

ACAT1 participates in the cholesterol metabolism pathway and the lipid biosynthesis pathway. It interacts with proteins such as LDLR (low-density lipoprotein receptor) by controlling the availability of free cholesterol within cells. These pathways are critical in maintaining lipid balance and influencing lipoprotein metabolism. ACAT1's enzymatic activity impacts how cells manage cholesterol influx and storage linking to broader lipid regulation networks.

Associated diseases and disorders

ACAT1 is associated with atherosclerosis and Alzheimer's disease. The conversion of cholesterol into cholesteryl esters affects the development of atherosclerotic plaques contributing to cardiovascular disease risk. In Alzheimer's disease ACAT1 may influence the accumulation of amyloid-beta a protein linked to the pathology of the disease. The protein APP (amyloid precursor protein) connects to ACAT1 as disturbances in cholesterol metabolism can affect amyloid-beta production linking this enzyme to neurodegenerative conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)
Lanes 1 - 4: Merged signal (red and green). Green - ab168342 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab168342 was shown to react with ACAT1 in western blot. The band observed in the CRISPR/Cas9 edited lysate lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab168342 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution
Lane 1: Wild-type SW480 cell lysate at 20 µg
Lane 2: ACAT1 CRISPR/Cas9 edited SW480 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
ab168342 showing +ve staining in Human ovarian carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)
All lanes: Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution
Lane 1: Human fetal liver lysates at 10 µg
Lane 2: HepG2 lysates at 10 µg
Lane 3: SKBR-3 lysates at 10 µg
Lane 4: THP-1 lysates at 10 µg
Lane 5: Human fetal kidney lysates at 10 µg
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
ab168342 showing +ve staining in Human normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)
Lanes 1 - 4: Merged signal (red and green). Green - ab168342 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab168342 was shown to react with ACAT1 in western blot. The band observed in the knockout cell line Human ACAT1 knockout SW480 cell line ab269621 (knockout cell lysate Human ACAT1 knockout SW480 cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab168342 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution
Lane 1: Wild-type SW480 cell lysate at 20 µg
Lane 2: ACAT1 knockout SW480 cell lysate at 20 µg
Lane 2: Western blot - Human ACAT1 knockout SW480 cell line (Human ACAT1 knockout SW480 cell line ab269621)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
ab168342 showing +ve staining in Human normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ACAT1 antibody [EPR10359] (ab168342)
Western blot analysis labelling ACAT1 on immunoprecipitation pellet from Human fetal liver lysate using ab168342 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-ACAT1 antibody [EPR10359] (ab168342)
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
ab168342 showing +ve staining in Human papillary adenocarcinoma of thyroid gland tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACAT1 with ab168342 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACAT1 with ab168342 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.