Anti-ACAT1 antibody [EPR10359]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

ab168342 showing +ve staining in Human normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACAT1 with ab168342 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

ab168342 showing +ve staining in Human papillary adenocarcinoma of thyroid gland tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACAT1 with ab168342 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

ab168342 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (AB168342)

ab168342 showing +ve staining in Human ovarian carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IP

Unknown

Immunoprecipitation - Anti-ACAT1 antibody [EPR10359] (AB168342)

Western blot analysis labelling ACAT1 on immunoprecipitation pellet from Human fetal liver lysate using ab168342 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-ACAT1 antibody [EPR10359] (ab168342)

Predicted band size: 45 kDa

false

• WB

Lab

Western blot - Anti-ACAT1 antibody [EPR10359] (AB168342)

Lanes 1 - 4 : Merged signal (red and green). Green - ab168342 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab168342 was shown to react with ACAT1 in western blot. The band observed in the CRISPR/Cas9 edited lysate lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab168342 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

Lane 1:

Wild-type SW480 cell lysate at 20 µg

Lane 2:

ACAT1 CRISPR/Cas9 edited SW480 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

• WB

Lab

Western blot - Anti-ACAT1 antibody [EPR10359] (AB168342)

Lanes 1 - 4 : Merged signal (red and green). Green - ab168342 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab168342 was shown to react with ACAT1 in western blot. The band observed in the knockout cell line ab269621 (knockout cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab168342 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

Lane 1:

Wild-type SW480 cell lysate at 20 µg

Lane 2:

ACAT1 knockout SW480 cell lysate at 20 µg

Lane 2:

Western blot - Human ACAT1 knockout SW480 cell line (<a href='/en-us/products/cell-lines/human-acat1-knockout-sw480-cell-line-ab269621'>ab269621</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

• WB

Unknown

Western blot - Anti-ACAT1 antibody [EPR10359] (AB168342)

All lanes:

Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

HepG2 lysates at 10 µg

Lane 3:

SKBR-3 lysates at 10 µg

Lane 4:

THP-1 lysates at 10 µg

Lane 5:

Human fetal kidney lysates at 10 µg

Predicted band size: 45 kDa

false