AB191431

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)]

Name: Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) | Abcam
Brand: Abcam Limited
SKU: AB191431
Price: 330 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal ACAT2/Acetyl-CoA acetyltransferase antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

View Alternative Names

ACTL, ACAT2, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase

3 Images

Supplier Data

Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab191431 at 1/20 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

All lanes:

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

All lanes:

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/1000 dilution

Lane 1:

PC12 cell lysate at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa

false

Carrier free

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8416(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAT2 also known as Acetyl-CoA acetyltransferase is an enzyme that plays a role in lipid metabolism catalyzing the conversion of two molecules of acetyl-CoA into acetoacetyl-CoA. It shares functionality with other acetyltransferases such as chloramphenicol acetyltransferase. ACAT2 has a molecular mass of approximately 41 kDa. Expression of ACAT2 is mainly in the liver and the cytosol of cells involved in sterol regulatory processes. It functions as a homotetramer and is essential in maintaining cellular levels of acetyl-CoA and related metabolites.

Biological function summary

This enzyme facilitates the synthesis of cholesterol and ketone bodies essential processes for cellular energy balance. ACAT2 works as an independent enzyme rather than as a component of a multi-enzyme complex unlike some other acetyltransferases. By managing acetyl-CoA levels it supports energy homeostasis and lipogenesis providing substrates for downstream processes that are critical for sustaining cellular function and response to energy demands.

Pathways

The acetyl-transfer reactions catalyzed by ACAT2 are integral to the cholesterol biosynthesis and ketogenesis pathways. These pathways are important for energy balance and lipid modification. Within these pathways ACAT2 acts in close relation with HMG-CoA synthase an important player in cholesterol biosynthesis. The enzyme’s actions provide acetoacetyl-CoA which is a substrate for the production of HMG-CoA highlighting its role in maintaining metabolic flux.

ACAT2's role in cholesterol metabolism links it to atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Disruption in its function can lead to elevated levels of cholesterol and triglycerides risk factors for atherosclerosis. In NAFLD ACAT2 expression may influence lipid accumulation in the liver contributing to disease pathogenesis. Its connection with other proteins like LDL receptor and apolipoprotein B through cholesterol metabolism emphasizes its potential as a therapeutic target for managing lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthetic pathway of cholesterol.

See full target information ACAT2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 12:713293 PubMed34664012

2021

Acetylation Profiles in the Metabolic Process of Glioma-Associated Seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Wen Xu,Peng Lin,Shu-Fa Zheng,Wen Huang,Zhang-Ya Lin,Huang-Cheng Shang-Guan,Yuan-Xiang Lin,Pei-Sen Yao,De-Zhi Kang

Endocrinology 158:3778-3791 PubMed28973134

2017

MEF2C Interacts With c-FOS in PTH-Stimulated Mmp13 Gene Expression in Osteoblastic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Teruyo Nakatani,Nicola C Partridge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)]

Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (AB191431)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise