AB131215

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417]

Name: Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) | Abcam
Brand: Abcam Limited
SKU: AB131215
Price: 305 USD
Availability: InStock
Rating: 5 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal ACAT2/Acetyl-CoA acetyltransferase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

ACTL, ACAT2, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

All lanes:

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa

false

OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8417

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 mins.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAT2 also known as Acetyl-CoA acetyltransferase is an enzyme that plays a role in lipid metabolism catalyzing the conversion of two molecules of acetyl-CoA into acetoacetyl-CoA. It shares functionality with other acetyltransferases such as chloramphenicol acetyltransferase. ACAT2 has a molecular mass of approximately 41 kDa. Expression of ACAT2 is mainly in the liver and the cytosol of cells involved in sterol regulatory processes. It functions as a homotetramer and is essential in maintaining cellular levels of acetyl-CoA and related metabolites.

Biological function summary

This enzyme facilitates the synthesis of cholesterol and ketone bodies essential processes for cellular energy balance. ACAT2 works as an independent enzyme rather than as a component of a multi-enzyme complex unlike some other acetyltransferases. By managing acetyl-CoA levels it supports energy homeostasis and lipogenesis providing substrates for downstream processes that are critical for sustaining cellular function and response to energy demands.

Pathways

The acetyl-transfer reactions catalyzed by ACAT2 are integral to the cholesterol biosynthesis and ketogenesis pathways. These pathways are important for energy balance and lipid modification. Within these pathways ACAT2 acts in close relation with HMG-CoA synthase an important player in cholesterol biosynthesis. The enzyme’s actions provide acetoacetyl-CoA which is a substrate for the production of HMG-CoA highlighting its role in maintaining metabolic flux.

ACAT2's role in cholesterol metabolism links it to atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Disruption in its function can lead to elevated levels of cholesterol and triglycerides risk factors for atherosclerosis. In NAFLD ACAT2 expression may influence lipid accumulation in the liver contributing to disease pathogenesis. Its connection with other proteins like LDL receptor and apolipoprotein B through cholesterol metabolism emphasizes its potential as a therapeutic target for managing lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthetic pathway of cholesterol.

See full target information ACAT2

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 19:7 PubMed38178203

2024

High-expressed ACAT2 predicted the poor prognosis of platinum-resistant epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfeng Wang,Zhe Yang,Han Bai,Lanbo Zhao,Jing Ji,Yadi Bin,Yu Liu,Siyi Zhang,Huilian Hou,Qiling Li

Molecular carcinogenesis 62:754-770 PubMed36920044

2023

Circular RNA ACACA negatively regulated p53-modulated mevalonate pathway to promote colorectal tumorigenesis via regulating miR-193a/b-3p/HDAC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjiao He,Qiong Liu,Huan Liu,Qian Pei,Hong Zhu

Antioxidants (Basel, Switzerland) 12: PubMed36978970

2023

Inhibiting NLRP3 Inflammasome Activation by CY-09 Helps to Restore Cerebral Glucose Metabolism in 3×Tg-AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangxue Han,Zhijun He,Xia Hu,Xiaoqian Li,Kaixin Zheng,Yingying Huang,Peng Xiao,Qingguo Xie,Jiazuan Ni,Qiong Liu

Journal of dairy science 105:7773-7786 PubMed35940922

2022

Role of sortilin 1 (SORT1) on fatty acid-mediated cholesterol metabolism in primary calf hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Wang,Qianming Jiang,Juan J Loor,Changhong Gao,Mingmao Yang,Yan Tian,Wenwen Fan,Bingbing Zhang,Ming Li,Chuang Xu,Wei Yang

Frontiers in cell and developmental biology 10:927118 PubMed35912092

2022

Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabetta Vergani,Giovanni L Beretta,Mariachiara Aloisi,Matteo Costantino,Cristina Corno,Simona Frigerio,Stella Tinelli,Matteo Dugo,Felice Maria Accattatis,Agnese Granata,Lorenzo Arnaboldi,Monica Rodolfo,Paola Perego,Laura Gatti

Journal of bioenergetics and biomembranes 53:415-428 PubMed34036483

2021

Circ_RPL23A acts as a miR-1233 sponge to suppress the progression of clear cell renal cell carcinoma by promoting ACAT2.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Cheng,Huifeng Cao,Jianbo Xu,Mo Xu,Wenjie He,Wenjing Zhang,Longxin Dong,Dayin Chen

Journal of hematology & oncology 14:70 PubMed33902674

2021

Targeting the metabolic vulnerability of acute myeloid leukemia blasts with a combination of venetoclax and 8-chloro-adenosine.

Applications

Unspecified application

Species

Unspecified reactive species

Ralf Buettner,Le Xuan Truong Nguyen,Corey Morales,Min-Hsuan Chen,Xiwei Wu,Lisa S Chen,Dinh Hoa Hoang,Servando Hernandez Vargas,Vinod Pullarkat,Varsha Gandhi,Guido Marcucci,Steven T Rosen

Molecular therapy. Nucleic acids 24:711-727 PubMed33996254

2021

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Zhang,Huiqing Wen,Bangjian Peng,Jun Weng,Fanhong Zeng

Yonsei medical journal 61:780-788 PubMed32882762

2020

MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Tian,Hui Min Ying,Yuan Yuan Wang,Bo Ning Cheng,Juan Chen

OncoTargets and therapy 13:3477-3488 PubMed32425549

2020

ACAT2 Promotes Cell Proliferation and Associates with Malignant Progression in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meilin Weng,Hao Zhang,Wenting Hou,Zhirong Sun,Jing Zhong,Changhong Miao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (AB131215)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise