AB248375

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ACAT2/Acetyl-CoA acetyltransferase antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ACTL, ACAT2, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

This data was developed using ab131215, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

This data was developed using ab131215, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (<a href='/en-us/products/primary-antibodies/acat2-acetyl-coa-acetyltransferase-antibody-epr8417-ab131215'>ab131215</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa

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OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

OI-RD Scanning

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OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8417

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248375 is the carrier-free version of ab131215.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAT2 also known as Acetyl-CoA acetyltransferase is an enzyme that plays a role in lipid metabolism catalyzing the conversion of two molecules of acetyl-CoA into acetoacetyl-CoA. It shares functionality with other acetyltransferases such as chloramphenicol acetyltransferase. ACAT2 has a molecular mass of approximately 41 kDa. Expression of ACAT2 is mainly in the liver and the cytosol of cells involved in sterol regulatory processes. It functions as a homotetramer and is essential in maintaining cellular levels of acetyl-CoA and related metabolites.

Biological function summary

This enzyme facilitates the synthesis of cholesterol and ketone bodies essential processes for cellular energy balance. ACAT2 works as an independent enzyme rather than as a component of a multi-enzyme complex unlike some other acetyltransferases. By managing acetyl-CoA levels it supports energy homeostasis and lipogenesis providing substrates for downstream processes that are critical for sustaining cellular function and response to energy demands.

Pathways

The acetyl-transfer reactions catalyzed by ACAT2 are integral to the cholesterol biosynthesis and ketogenesis pathways. These pathways are important for energy balance and lipid modification. Within these pathways ACAT2 acts in close relation with HMG-CoA synthase an important player in cholesterol biosynthesis. The enzyme’s actions provide acetoacetyl-CoA which is a substrate for the production of HMG-CoA highlighting its role in maintaining metabolic flux.

ACAT2's role in cholesterol metabolism links it to atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Disruption in its function can lead to elevated levels of cholesterol and triglycerides risk factors for atherosclerosis. In NAFLD ACAT2 expression may influence lipid accumulation in the liver contributing to disease pathogenesis. Its connection with other proteins like LDL receptor and apolipoprotein B through cholesterol metabolism emphasizes its potential as a therapeutic target for managing lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthetic pathway of cholesterol.

See full target information ACAT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2020:7019597 PubMed33123597

2020

Regulation and Mechanism of miR-518d through the PPAR-Mediated NF-B Pathway in the Development of Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Qiu,Xuemin Liu,Shenshen Yao,Jiaren Zhou,Xue Zhang,Juan Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (AB248375)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise