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Rabbit Recombinant Monoclonal ACBD3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.


Images

Western blot - Anti-ACBD3 antibody [EPR8623] (AB134952), expandable thumbnail
  • Western blot - Anti-ACBD3 antibody [EPR8623] (AB134952), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] (AB134952), expandable thumbnail
  • OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] (AB134952), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi (PubMed:11590181). Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity). Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase (PubMed:27009356). (Microbial infection) Plays an essential role in Aichi virus RNA replication by recruiting PI4KB at the viral replication sites.

Alternative names

GCP60, GOCAP1, GOLPH1, ACBD3, Golgi resident protein GCP60, Acyl-CoA-binding domain-containing protein 3, Golgi complex-associated protein 1, Golgi phosphoprotein 1, PBR- and PKA-associated protein 7, Peripheral benzodiazepine receptor-associated protein PAP7

Recommended products

Rabbit Recombinant Monoclonal ACBD3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8623
Purity
Tissue culture supernatant
Dissociation constant
2.28 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACBD3 also known by its alternate name GCP60 is a protein weighing approximately 60 kDa. This protein expresses in multiple tissues with significant presence in the Golgi apparatus where it plays a major role in maintaining Golgi structure and trafficking. ACBD3 helps anchor various proteins to the Golgi membranes facilitating essential cellular processes. It operates as a scaffold protein interacting with other proteins to regulate cellular localization and transport activities efficiently.

Biological function summary

ACBD3 plays a critical part in various cellular functions beyond just Golgi apparatus maintenance. It closely associates with other proteins to form part of larger functional networks such as the polypeptide N-acetylgalactosaminyltransferase enzyme complex. Through these interactions ACBD3 influences processes like lipid metabolism protein glycosylation and sterol biosynthesis. Its action is tied to the coordination of intracellular signaling and organization making it an important player in diverse cellular mechanisms.

Pathways

ACBD3 contributes significantly to the Golgi-ER transport pathway. This pathway is essential for protein sorting and trafficking impacting how proteins are directed and modified. ACBD3 interacts with proteins like PI4KB and GOLGA7 important for phosphoinositide signaling which regulates membrane trafficking and signaling at the Golgi. These pathways connect to broader cellular networks and ensure proper function and organization within the cell.

Associated diseases and disorders

ACBD3 has links with several conditions highlighting its functional significance. Altered ACBD3 activity associates with neurodegenerative diseases and cancers. It interacts with proteins such as Coxsackievirus and Adenovirus Receptor (CAR) in viral infections illustrating its role in disease mechanisms. These interactions highlight the potential of ACBD3 as a target for therapeutic intervention or a biomarker in disease diagnosis and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952), expandable thumbnail

    Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: HepG2 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab134952 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab134952 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. ab134952 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)

    Predicted band size: 61 kDa

  • Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952), expandable thumbnail

    Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952)

    All lanes: Western blot - Anti-ACBD3 antibody [EPR8623] (ab134952) at 1/1000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 4: LNCaP cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 61 kDa

  • Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] (ab134952), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ACBD3 antibody [EPR8623] (ab134952)

    Intracellular flow cytometric analysis of permeabilized K562 cells labelling ACBD3 using ab134952 (red) at 1/100 dilution and rabbit IgG negative control (green).

  • OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] (ab134952), expandable thumbnail

    OI-RD Scanning - Anti-ACBD3 antibody [EPR8623] (ab134952)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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