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AB112050

Anti-ACD antibody

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(2 Publications)

Rabbit Polyclonal ACD antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ACD aa 150-200.

View Alternative Names

PIP1, PTOP, TINT1, TPP1, ACD, Adrenocortical dysplasia protein homolog, POT1 and TIN2-interacting protein

2 Images
Immunoprecipitation - Anti-ACD antibody (AB112050)
  • IP

Unknown

Immunoprecipitation - Anti-ACD antibody (AB112050)

ab112050 at 1 µg/ml staining PDE3A in HeLa cell lysate immunoprecipitated using ab112050 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : controle IgG. Predicted band size : 58 kDa Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-ACD antibody (ab112050)

Predicted band size: 58 kDa

false

Western blot - Anti-ACD antibody (AB112050)
  • WB

Unknown

Western blot - Anti-ACD antibody (AB112050)

All lanes:

Western blot - Anti-ACD antibody (ab112050) at 0.4 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 5 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 58 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ACD aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q96AP0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ACD also known as TPP1 is a component of the shelterin complex which protects telomeres. Its molecular mass is approximately 55 kDa. ACD is expressed mainly in the nucleus of cells where it interacts with telomeric DNA. ACD plays a role in regulating telomere length and stability contributing to cellular replication and aging processes.
Biological function summary

ACD interacts with other proteins within the shelterin complex such as POT1 and TRF1. This complex is critical in protecting chromosome ends from DNA damage responses. ACD is important for maintaining telomere integrity supporting cellular proliferation. When ACD functions properly it helps prevent chromosomal instability and potential oncogenic transformations.

Pathways

ACD participates in the telomere maintenance pathway and the DNA damage response pathway. ACD closely associates with proteins like POT1 and TRF2 in these pathways. Through these interactions ACD assists in modulating telomerase activity which is essential for balancing telomere elongation and degradation.

Disruptions in ACD function have links to conditions like dyskeratosis congenita and some cancers. ACD malfunction contributes to telomere shortening leading to premature aging and increased cancer risk. It associates with proteins like TERT in these contexts where imbalances can exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity. May play a role in organogenesis.
See full target information ACD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3493 PubMed35715464

2022

ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Binbin Ma,Xiaoxu Liu,Ge Gao,Zhuanzhuan Che,Menghan Fan,Siyan Meng,Xiru Zhao,Rio Sugimura,Hua Cao,Zhongjun Zhou,Jing Xie,Chengqi Lin,Zhuojuan Luo

PloS one 17:e0264073 PubMed35176105

2022

Insights into POT1 structural dynamics revealed by cryo-EM.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel W Smith,Simon Lattmann,Zhehui Barry Liu,Bilal Ahsan,Daniela Rhodes
View all publications
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Product promise

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