Anti-ACE2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (AB15348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (AB15348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.

Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (AB15348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.

Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (AB15348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (AB15348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• WB

Lab

Western blot - Anti-ACE2 antibody (AB15348)

False colour image of Western blot : Anti-ACE2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15348 was shown to bind specifically to ACE2. A band was observed at 120-135 kDa in wild-type Caco-2 cell lysates with no signal observed at this size in ACE2 knockout cell line ab277338 (knockout cell lysate ab275516). To generate this image, wild-type and ACE2 knockout Caco-2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-ACE2 antibody (ab15348) at 1/1000 dilution

Lane 1:

Wild-type Caco-2 cell lysate at 30 µg

Lane 2:

ACE2 knockout Caco-2 cell lysate at 30 µg

Lane 3:

Calu-3 cell lysate at 30 µg

Lane 4:

A549 cell lysate at 30 µg

Predicted band size: 92 kDa

Observed band size: 120-135 kDa

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• WB

Supplier Data

Western blot - Anti-ACE2 antibody (AB15348)

All lanes:

Western blot - Anti-ACE2 antibody (ab15348) at 2 µg/mL

Lane 1:

Human Testis Lysate at 15 µg

Lane 2:

Human Lung lysate at 15 µg

Lane 3:

Human intestine lysate at 15 µg

Lane 4:

Human Breast lysate at 15 µg

Lane 5:

Caco2 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 125 kDa

false

• WB

Unknown

Western blot - Anti-ACE2 antibody (AB15348)

Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).

Lane 1:

Western blot - Anti-ACE2 antibody (ab15348) at 0.5 µg/mL

Lane 2:

Western blot - Anti-ACE2 antibody (ab15348) at 1 µg/mL

Lane 3:

Western blot - Anti-ACE2 antibody (ab15348) at 2 µg/mL

All lanes:

Human Kidney Lysate

Predicted band size: 92 kDa

Observed band size: 105 kDa

false

• WB

Supplier Data

Western blot - Anti-ACE2 antibody (AB15348)

Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACE2 antibody (ab15348) at 2 µg/mL

Lane 1:

Mouse liver tissue lysate at 15 µg

Lane 2:

Mouse spleen tissue lysate at 15 µg

Lane 3:

Mouse heart tissue lysate at 15 µg

Lane 4:

Mouse bladder tissue lysate at 15 µg

Lane 5:

Mouse pancreas tissue lysate at 15 µg

Lane 6:

Mouse stomach tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

• WB

Supplier Data

Western blot - Anti-ACE2 antibody (AB15348)

Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-ACE2 antibody (ab15348) at 2 µg/mL

Lane 1:

Rat skin tissue lysate at 15 µg

Lane 2:

Rat spleen tissue lysate at 15 µg

Lane 3:

Rat heart tissue lysate at 15 µg

Lane 4:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 125 kDa

false