Anti-ACE2 antibody [EPR24705-45] (ab272500) is a rabbit monoclonal antibody that is used to detect ACE2 in Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.
- Specificity confirmed with ACE2 knockout cell line validation
Images
![Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (AB272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--flow-cytometry-img180012.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (AB272500)")
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img170960.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (AB272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184384.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (AB272500)")
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img165582.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500)")
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img167931.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (AB272500)")
Publications
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- Journal of virology 97:e00555232023One for all-human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison Daniels,Sarah Fletcher,Holly E M Kerr,Annika Kratzel,Rute Maria Pinto,Nisha Kriplani,Nicky Craig,C James Hastie,Paul Davies,Paul Digard,Volker Thiel,Christine Tait-BurkardPubMed 37668370
- Nanoscale advances 5:4167-41812023SARS-CoV-2 virus-like-particles liposomal reconstitution of spike glycoproteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah McColman,Klaidi Shkalla,Pavleen Sidhu,Jady Liang,Selena Osman,Norbert Kovacs,Zainab Bokhari,Ana Carolina Forjaz Marques,Yuchong Li,Qiwen Lin,Haibo Zhang,David T CrambPubMed 37560413
- Research (Washington, D.C.) 6:01242023SARS-CoV-2 RBD and Its Variants Can Induce Platelet Activation and Clearance: Implications for Antibody Therapy and Vaccinations against COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoying Ma,Jady Liang,Guangheng Zhu,Preeti Bhoria,Aron A Shoara,Daniel T MacKeigan,Christopher J Khoury,Sladjana Slavkovic,Lisha Lin,Danielle Karakas,Ziyan Chen,Viktor Prifti,Zhenze Liu,Chuanbin Shen,Yuchong Li,Cheng Zhang,Jiayu Dou,Zack Rousseau,Jiamin Zhang,Tiffany Ni,Xi Lei,Pingguo Chen,Xiaoyu Wu,Hamed Shaykhalishahi,Samira Mubareka,Kim A Connelly,Haibo Zhang,Ori Rotstein,Heyu NiPubMed 37223472
- Nature communications 13:79072022Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanming Guo,Jianli Cao,Jian-Piao Cai,Jiayan Wu,Jiangang Huang,Pallavi Asthana,Sheung Kin Ken Wong,Zi-Wei Ye,Susma Gurung,Yijing Zhang,Sheng Wang,Zening Wang,Xin Ge,Hiu Yee Kwan,Aiping Lyu,Kui Ming Chan,Nathalie Wong,Jiandong Huang,Zhongjun Zhou,Zhao-Xiang Bian,Shuofeng Yuan,Hoi Leong Xavier WongPubMed 36564389
- Frontiers in immunology 13:8271462022SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Perico,Marina Morigi,Miriam Galbusera,Anna Pezzotta,Sara Gastoldi,Barbara Imberti,Annalisa Perna,Piero Ruggenenti,Roberta Donadelli,Ariela Benigni,Giuseppe RemuzziPubMed 35320941
- Experimental eye research 214:1088642021Hydroxychloroquine treatment on SARS-CoV-2 receptor ACE2, TMPRSS2 and NRP1 expression in human primary pterygium and conjunctival cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Yao,Di Ma,Yanxuan Xu,Xiang-Ling Yuan,Jia-Jian Liang,Shao-Lang Chen,Vishal Jhanji,Tsz Kin NgPubMed 34826419
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2. Non-functional as a carboxypeptidase. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Alternative names
UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
Recommended products
Anti-ACE2 antibody [EPR24705-45] (ab272500) is a rabbit monoclonal antibody that is used to detect ACE2 in Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human samples.
- Specificity confirmed with ACE2 knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24705-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ACE2 antibody [EPR24705-45] (ab272500) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-ACE2 antibody [EPR24705-45] (ab272500) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in ACE2 Knockout CACO-2 cell line, Human ACE2 knockout Caco-2 cell line ab273731.
Other related products
We have a range of other formats of antibody clone [EPR24705-45] also available for your convenience:
ab272500, Carrier free - Anti-ACE2 antibody [EPR24705-45] - BSA and Azide free ab282118, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-ACE2 antibody [EPR24705-45] ab283658
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
- Biological function summary
The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.
- Pathways
ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.
- Associated diseases and disorders
ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--flow-cytometry-img180012.jpg "Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left)/ Calu-3 (human lung adenocarcinoma epithelial cell, Right) cells labelling ACE2 with ab272500 at 1/500 dilution (0.1ug). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 32926098). Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img170960.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
ab272500 staining ACE2 in wild-type Caco2 cells (top panel) and ACE2 knockout Caco2 cells (Human ACE2 knockout Caco-2 cell line ab273731) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab272500 at 10μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184384.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous and cytoplasmic staining in human testis. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img165582.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling ACE2 with ab272500 at 1/250 (2.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with myc-tagged ACE2 expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunocytochemistry-immunofluorescence-img167931.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Calu-3 cells labelling ACE2 with ab272500 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of Calu-3 cells. Negative control: HeLa (PMID: 32926098). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Red) was used at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178802.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human kidney. The section was incubated with ab272500 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167326.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human liver. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178802.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-epr24705-45-ab272500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167326.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)")
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