Name: Anti-ACE2 antibody [EPR4435(2)]
SKU: ab108252
Rating: 4 (10 reviews)

Anti-ACE2 antibody [EPR4435(2)] (ab108252) is a rabbit monoclonal antibody that is used to detect ACE2 in Western Blot, IP, IHC-P, ELISA. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with ACE2 knockout cell line validation

Related conjugates and formulations

ab313253
Conjugated
Alexa Fluor® 555 Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)]
ab311772
Conjugated
Alexa Fluor® 594 Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)]
ab311135
Conjugated
Alexa Fluor® 647 Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)]
ab239924
Carrier free
Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free
ab317075
Conjugated
Alexa Fluor® 488 Anti-ACE2 antibody [EPR4435(2)]

Images

Western blot - Anti-ACE2 antibody [EPR4435(2)] (AB108252), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	I-ELISA	WB	ICC/IF	Flow Cyt
Human	Tested	Tested	Expected	Tested	Not recommended	Not recommended
Mouse	Tested	Expected	Expected	Tested	Not recommended	Not recommended
Rat	Tested	Expected	Expected	Tested	Not recommended	Not recommended
Synthetic peptide	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/6400 - 1/32000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/6400 - 1/32000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/6400 - 1/32000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Synthetic peptide	Dilution info -	Notes -

Associated Products

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Target data

See full target information ACE2

Function

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2. Non-functional as a carboxypeptidase. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.

Alternative names

UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4435(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-ACE2 antibody [EPR4435(2)] (ab108252) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA, in Human, Mouse, Rat samples.

What is the molecular weight of ACE2?

Anti-ACE2 [EPR4435(2)] (ab108252) specifically detects a band for ACE2 (UniProt: Q9BYF1) at a molecular weight of 92kDa.

Recommended positive controls

WB: Human testis, kidney and lung tissue lysates; Human fetal kidney tissue lysate; Calu-3, HepG2 and Caco-2 cell lysates. Human and rat heart tissue lysate; Human lung tissue lysate; Mouse and rat spleen, testis lung tissue lysate;IHC-P: Human, mouse, and rat kidney tissues.IP: Human testis tissue lysate.

Trusted by the scientific community

Anti-ACE2 [EPR4435(2)] (ab108252) was first used in a scientific publication in 2011 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists

Anti-ACE2 [EPR4435(2)] (ab108252) has over 10 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed

The specificity of Anti-ACE2 antibody [EPR4435(2)] (ab108252) has been confirmed by Western blot testing in ACE2 Knockout HepG2 cell line, ab273733.

Other related products

We have a range of other formats of antibody clone [EPR4435(2)] also available for your convenience:
ab108252, Carrier free - ab239924, Alexa Fluor® 647 - ab311135, Alexa Fluor® 594 - ab311772, Alexa Fluor® 555 - ab313253, Alexa Fluor® 488 - ab317075

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.

Biological function summary

The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.

Pathways

ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.

Associated diseases and disorders

ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108252 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line Human ACE2 knockout Hep G2 cell line ab273733 (knockout cell lysate Human ACE2 knockout Hep G2 cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108252 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Wild-type HepG2 cell lysate at 30 µg
Lane 2: ACE2 knockout HepG2 cell lysate at 30 µg
Lane 3: Calu-3 cell lysate at 30 µg
Lane 4: A549 cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The section was incubated with ab108252 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 125 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108252 was shown to react with ACE2 in Caco-2 wild-type cells in western blot with loss of signal observed in ACE2 knockout cell line Human ACE2 knockout Caco-2 cell line ab273731 (knockout cell lysate Human ACE2 knockout Caco-2 cell lysate ab275516). Wild-type and ACE2 knockout Caco-2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108252 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Wild-type Caco-2 cell lysate at 30 µg
Lane 2: ACE2 knockout Caco-2 cell lysate at 30 µg
Lane 2: Western blot - Human ACE2 knockout Caco-2 cell line (Human ACE2 knockout Caco-2 cell line ab273731)
Lane 3: Calu-3 cell lysate at 30 µg
Lane 4: A549 cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 104 kDa, 60 kDa, 76 kDa, 92 kDa
Observed band size: 125 kDa, 75 kDa
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Lanes 1 - 6: Merged signal (red and green). Green - ab108252 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.
ab108252 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108252 and Mouse anti Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Human testis cell lysate at 20 µg
Lane 2: Human kidney cell lysate at 20 µg
Lane 3: Human lung cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Lane 5: Caco-2 cell lysate at 20 µg
Lane 6: A549 cell lysate (negative control) at 20 µg
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 120 kDa
Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
ab108252 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 0.6 μg primary antibody (1/20). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000) was used to confirm successful immunoprecipitation.
Exposure time: 1 second.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/500 dilution
Lane 1: Human testis tissue lysate at 10 µg
Lane 2: ab108252 + Human testis tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108252 in Human testis tissue lysate
Predicted band size: 92 kDa
Observed band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The section was incubated with ab108252 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
The section was incubated with ab108252 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Different batches of ab108252 were tested on Human kidney lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Predicted band size: 92 kDa
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: Human testis lysate at 10 µg
Predicted band size: 92 kDa
Indirect ELISA - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
ELISA using ab108252 at varying antibody concentrations (4000~0 ng/ml) and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
ACE2 Western blot staining using rabbit Anti-ACE2 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

Two bands observed by ab108252 corresponding to glycosylation and non-glycosylation forms.

Signal in heart tissue is low, we recommend loading more amount of lysate or using lower antibody dilution to improve result.
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg
Lane 2: Rat heart tissue lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 110 kDa, 120 kDa
Exposure time: 180s
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
ACE2 Western blot staining using rabbit Anti-ACE2 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.

Exposure time: Lane 1: 7 seconds; Lane 2-8: 180 seconds.

Two bands observed by ab108252 corresponding to glycosylation and non-glycosylation forms.

Signal in mouse and rat tissues are low, we recommend loading more amount of lysate or using lower antibody dilution to improve result.
All lanes: Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human lung tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse spleen tissue lysate
Lane 5: Mouse lung tissue lysate
Lane 6: Rat testis tissue lysate
Lane 7: Rat spleen tissue lysate
Lane 8: Rat lung tissue lysate
Secondary
All lanes: Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 110 kDa, 120 kDa