Mouse Monoclonal ACE2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACE2.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (AB89111), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-mm0073-11a31-ab89111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (AB89111)")
Publications
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- Viruses 14:2022SARS-CoV-2, Placental Histopathology, Gravity of Infection and Immunopathology: Is There an Association?Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonardo Resta,Antonella Vimercati,Gerardo Cazzato,Margherita Fanelli,Sara Vincenza Scarcella,Giuseppe Ingravallo,Anna Colagrande,Sara Sablone,Mary Stolfa,Francesca Arezzo,Teresa Lettini,Roberta RossiPubMed 35746801
- Human vaccines & immunotherapeutics 18:20486222022Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeodor-D Brumeanu,Pooja Vir,Ahmad Faisal Karim,Swagata Kar,Dalia Benetiene,Megan Lok,Jack Greenhouse,Tammy Putmon-Taylor,Christopher Kitajewski,Kevin K Chung,Kathleen P Pratt,Sofia A CasaresPubMed 35348437
- International journal of chronic obstructive pulmonary disease 17:101-1152022SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD - Possible Implications for Interstitial Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel James Brake,Mathew Suji Eapen,Kielan Darcy McAlinden,James Markos,Greg Haug,Josie Larby,Collin Chia,Ashutosh Hardikar,Gurpreet Kaur Singhera,Tillie L Hackett,Wenying Lu,Sukhwinder Singh SohalPubMed 35046647
- JCI insight 6:2021L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuji Kondo,Jason L Larabee,Liang Gao,Huiping Shi,Bojing Shao,Christopher M Hoover,J Michael McDaniel,Yen-Chun Ho,Robert Silasi-Mansat,Stephanie A Archer-Hartmann,Parastoo Azadi,R Sathish Srinivasan,Alireza R Rezaie,Alain Borczuk,Jeffrey C Laurence,Florea Lupu,Jasimuddin Ahamed,Rodger P McEver,James F Papin,Zhongxin Yu,Lijun XiaPubMed 34291736
- Journal of clinical medicine 10:2021Electronic Cigarette Aerosol Is Cytotoxic and Increases ACE2 Expression on Human Airway Epithelial Cells: Implications for SARS-CoV-2 (COVID-19).Applications:Unspecified applicationReactive species:Unspecified reactive speciesKielan Darcy McAlinden,Wenying Lu,Parisa Vahidi Ferdowsi,Stephen Myers,James Markos,Josie Larby,Collin Chia,Heinrich C Weber,Greg Haug,Mathew Suji Eapen,Sukhwinder Singh SohalPubMed 33802256
- Experimental eye research 205:1085272021Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRita Mencucci,Eleonora Favuzza,Matteo Becatti,Alessia Tani,Costanza Mazzantini,Roberto Vignapiano,Claudia Fiorillo,DomenicoE Pellegrini-Giampietro,Mirko Manetti,Mirca Marini,Elisa LanducciPubMed 33667466
- Zoological research 41:621-6312020Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an hACE2 transfection mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Liang,Heng Li,Jing Li,Ze-Ning Yang,Jia-Li Li,Hui-Wen Zheng,Yan-Li Chen,Hai-Jing Shi,Lei Guo,Long-Ding LiuPubMed 33045777
Key facts
- Isotype
- IgG2
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ACE2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYF1
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2. Non-functional as a carboxypeptidase. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Alternative names
UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
Recommended products
Mouse Monoclonal ACE2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACE2.
Key facts
- Isotype
- IgG2
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ACE2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYF1
- Clone number
- MM0073-11A31
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
- Biological function summary
The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.
- Pathways
ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.
- Associated diseases and disorders
ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111), expandable thumbnail](https://content.abcam.com/products/images/ace2-antibody-mm0073-11a31-ab89111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [MM0073-11A31] (ab89111)
IHC image of ACE2 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab89111, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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