Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)

Rabbit Recombinant Monoclonal ACACA antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (AB269272), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Not recommended	Tested	Tested
Mouse	Tested	Tested	Tested	Not recommended	Expected	Tested
Rat	Expected	Tested	Expected	Not recommended	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes -
Species Human	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information ACACA

Function

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443).

Alternative names

ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

Recommended products

Rabbit Recombinant Monoclonal ACACA antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23235-47
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Acetyl Coenzyme A Carboxylase Alpha also known as ACC1 is an important enzyme in fatty acid metabolism. It catalyzes the carboxylation of acetyl CoA to form malonyl CoA a critical step in fatty acid synthesis. ACC1 is a large protein with a mass of approximately 265 kDa and is mainly expressed in lipogenic tissues such as liver and adipose tissue. Additional isoforms have been identified with distinct tissue distribution patterns which allow for the regulation of fatty acid metabolism across different tissues.

Biological function summary

Acetyl Coenzyme A Carboxylase Alpha controls the availability of malonyl CoA serving as an important regulator of de novo lipogenesis. It functions as part of a homodimeric complex which is necessary for its optimal enzymatic activity. This regulation contributes to the balance of energy storage and consumption in cells. ACC1 not only facilitates the synthesis of long-chain fatty acids but also coordinates with other cellular processes that depend on lipid availability.

Pathways

ACC1 plays a central role in the fatty acid biosynthesis and energy metabolism pathways. It acts in concert with fatty acid synthase to promote production of fatty acids. ACC1's activity is modulated by several factors including AMP-activated protein kinase (AMPK) which phosphorylates ACC1 leading to its inhibition when energy levels are low. This positioning within the metabolism pathways makes ACC1 a critical point for controlling lipid biosynthesis in response to nutritional and hormonal signals.

Associated diseases and disorders

Alterations in the activity of Acetyl Coenzyme A Carboxylase Alpha are implicated in conditions such as obesity and type 2 diabetes. Dysregulation of ACC1 leads to aberrant lipid accumulation in tissues contributing to obesity-related insulin resistance. Furthermore the link between ACC1 and fatty acid metabolism associates it with metabolic syndromes. The potential of ACC1 as a pharmacological target has led to research exploring its inhibition as a therapeutic strategy for these metabolic disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell), whole cell lysate 10 μg with ab269272 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T whole cell lysate 10 μg.
Lane 2: ab269272 IP in 293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab269272 in 293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
All lanes: Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Predicted band size: 265 kDa
Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 μg with ab269272 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 whole cell lysate 10 μg.
Lane 2: ab269272 IP in C2C12 whole cell lysate 10 μg.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab269272 in C2C12 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
All lanes: Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Predicted band size: 265 kDa
Western blot - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 4: 48 seconds; Lane 2, 3: 26 seconds.
All lanes: Western blot - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272) at 1/5000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell), whole cell lysate, in the loading buffer containing double DTT at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, in the loading buffer containing double DTT at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate, in the loading buffer containing double DTT at 20 µg
Lane 4: C2C12 (mouse myoblasts myoblast), whole cell lysate, in the loading buffer containing double DTT at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 265 kDa
Observed band size: 265 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2 μg/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2 μg/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human endometrial carcinoma. The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human breast carcinoma (PMID: 21415164). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] (ab269272)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.