Rabbit Recombinant Monoclonal ACACA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23236-21] - BSA and Azide free (Detector) (AB285148), expandable thumbnail](https://content.abcam.com/products/images/acetyl-coenzyme-a-carboxylase-alpha-antibody-epr23236-21-bsa-and-azide-free-detector-ab285148--sandwich-elisa-img409348.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23236-21] - BSA and Azide free (Detector) (AB285148)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443).
Alternative names
ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha
Recommended products
Rabbit Recombinant Monoclonal ACACA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23236-21
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab285148 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat Acetyl Coenzyme A carboxylase alpha. The recommended pair for sandwich ELISA is:
Capture: Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-129] - BSA and Azide free (Capture) ab285042, Human/Mouse/Rat Acetyl Coenzyme A carboxylase alpha Capture Antibody (unconjugated)
Detector: ab285148, Human/Mouse/Rat Acetyl Coenzyme A carboxylase alpha Detector Antibody (unconjugated)
The reference range value is 1.56 - 100 ng/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetyl Coenzyme A Carboxylase Alpha also known as ACC1 is an important enzyme in fatty acid metabolism. It catalyzes the carboxylation of acetyl CoA to form malonyl CoA a critical step in fatty acid synthesis. ACC1 is a large protein with a mass of approximately 265 kDa and is mainly expressed in lipogenic tissues such as liver and adipose tissue. Additional isoforms have been identified with distinct tissue distribution patterns which allow for the regulation of fatty acid metabolism across different tissues.
- Biological function summary
Acetyl Coenzyme A Carboxylase Alpha controls the availability of malonyl CoA serving as an important regulator of de novo lipogenesis. It functions as part of a homodimeric complex which is necessary for its optimal enzymatic activity. This regulation contributes to the balance of energy storage and consumption in cells. ACC1 not only facilitates the synthesis of long-chain fatty acids but also coordinates with other cellular processes that depend on lipid availability.
- Pathways
ACC1 plays a central role in the fatty acid biosynthesis and energy metabolism pathways. It acts in concert with fatty acid synthase to promote production of fatty acids. ACC1's activity is modulated by several factors including AMP-activated protein kinase (AMPK) which phosphorylates ACC1 leading to its inhibition when energy levels are low. This positioning within the metabolism pathways makes ACC1 a critical point for controlling lipid biosynthesis in response to nutritional and hormonal signals.
- Associated diseases and disorders
Alterations in the activity of Acetyl Coenzyme A Carboxylase Alpha are implicated in conditions such as obesity and type 2 diabetes. Dysregulation of ACC1 leads to aberrant lipid accumulation in tissues contributing to obesity-related insulin resistance. Furthermore the link between ACC1 and fatty acid metabolism associates it with metabolic syndromes. The potential of ACC1 as a pharmacological target has led to research exploring its inhibition as a therapeutic strategy for these metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23236-21] - BSA and Azide free (Detector) (ab285148), expandable thumbnail](https://content.abcam.com/products/images/acetyl-coenzyme-a-carboxylase-alpha-antibody-epr23236-21-bsa-and-azide-free-detector-ab285148--sandwich-elisa-img409348.jpg "Sandwich ELISA - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23236-21] - BSA and Azide free (Detector) (ab285148)")
Sandwich ELISA - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23236-21] - BSA and Azide free (Detector) (ab285148)
Example of Acetyl Coenzyme A carboxylase alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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