JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109368

Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal ACACA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

8 Images
Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • WB

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109368 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab109368 was shown to specifically react with Acetyl Coenzyme A carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. ab109368 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Hela whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 266 kDa

false

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • WB

Unknown

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 266 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunofluorescent staining of 293 cells using unpurified ab109368 at 1/100 dilution

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunocytochemistry/ Immunofluorescence analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Acetyl Coenzyme A carboxylase with Purified ab109368 at 1 : 250 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Acetyl Coenzyme A carboxylase with purified ab109368 at 1 : 500 dilution (1.05 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer pH6.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemical analysis of paraffin-embedded brain tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)
  • WB

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

5% NFDM/TBST

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/5000 dilution

Lane 1:

293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 266 kDa

Observed band size: 266 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

  • 578 PE

    PE Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

  • 660 APC

    APC Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

  • Carrier free

    Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4971

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000- 1/10000.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000- 1/10000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000- 1/10000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB65341

Free Fatty Acid Assay Kit - Quantification

5

1 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.
Biological function summary

Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.

Pathways

Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.

Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443).
See full target information ACACA
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 23:83 PubMed37120513

2023

Imaging the metabolic reprograming of fatty acid synthesis pathway enables new diagnostic and therapeutic opportunity for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fukai Wang,Shuangshuang Ma,Panpan Chen,Yuhao Han,Zhaoyun Liu,Xinzhao Wang,Chenglong Sun,Zhiyong Yu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng

Medical oncology (Northwood, London, England) 39:202 PubMed36175596

2022

CD36 accelerates the progression of hepatocellular carcinoma by promoting FAs absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Lide Tao,Xiangmin Ding,Lele Yan,Guangcai Xu,Peijian Zhang,Anlai Ji,Lihong Zhang

Frontiers in bioengineering and biotechnology 10:858558 PubMed35769097

2022

LncRNA MALAT1 Promotes PPARα/CD36-Mediated Hepatic Lipogenesis in Nonalcoholic Fatty Liver Disease by Modulating miR-206/ARNT Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xiang,Yuan-Yuan Deng,Hui-Xia Liu,Ying Pu

Nature communications 13:1172 PubMed35246531

2022

Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Tao Zhang,Tao Ren,Xiaoyu Liao,Yilong Hao,Je Sun Lim,Jong-Ho Lee,Mi Li,Jichun Shao,Rui Liu

International journal of oncology 59: PubMed34533201

2021

LINC00514 promotes lipogenesis and tumor progression in esophageal squamous cell carcinoma by sponging miR‑378a‑5p to enhance SPHK1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Hongtao Liu,Qing Zhang,Xueying Zhang,Yue Qin,Guangzhao Zhu,Jinghan Dang,Feng Wang,Xiangxiang Yang,Ruitai Fan

Experimental and therapeutic medicine 21:560 PubMed33850532

2021

Adropin inhibits the phenotypic modulation and proliferation of vascular smooth muscle cells during neointimal hyperplasia by activating the AMPK/ACC signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Liang-Ping Zhao,Yu-Qi Chen,Xian-Song Chang,Hui Xiong,Dai-Min Zhang,Wei-Ting Xu,Jian-Chang Chen

Nutrients 12: PubMed32443556

2020

Bromelain Confers Protection against the Non-Alcoholic Fatty Liver Disease in Male C57bl/6 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Po-An Hu,Chia-Hui Chen,Bei-Chia Guo,Yu Ru Kou,Tzong-Shyuan Lee
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB222774

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270]

primary-antibodies

acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-rm270-ab222774

0

(0 reviews)

Alternative Product
Primary Antibodies

AB68191

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]

primary-antibodies

acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-ab68191

5

(1 reviews)

Alternative Product
Primary Antibodies

AB45174

Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

primary-antibodies

acetyl-coenzyme-a-carboxylase-antibody-ep687y-ab45174

4

(3 reviews)

Alternative Product
Primary Antibodies

AB173584

Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] - BSA and Azide free

primary-antibodies

acetyl-coenzyme-a-carboxylase-antibody-ep687y-bsa-and-azide-free-ab173584

0

(0 reviews)

Alternative Product
Primary Antibodies

AB72046

Anti-Acetyl Coenzyme A Carboxylase antibody

primary-antibodies

acetyl-coenzyme-a-carboxylase-antibody-ab72046

5

(2 reviews)

Alternative Product
Primary Antibodies

AB205883

Anti-Acetyl Coenzyme A Carboxylase antibody [143] - C-terminal

primary-antibodies

acetyl-coenzyme-a-carboxylase-antibody-143-c-terminal-ab205883

0

(0 reviews)

Alternative Product
Primary Antibodies

AB173583

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free

primary-antibodies

acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-bsa-and-azide-free-ab173583

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com