Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Acetyl Coenzyme A carboxylase with purified ab109368 at 1 : 500 dilution (1.05 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Acetyl Coenzyme A carboxylase with Purified ab109368 at 1 : 250 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 293 cells using unpurified ab109368 at 1/100 dilution

• WB

Unknown

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (<a href='/en-us/products/primary-antibodies/acetyl-coenzyme-a-carboxylase-antibody-epr4971-ab109368'>ab109368</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 266 kDa

false

• WB

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab109368 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab109368 was shown to specifically react with Acetyl Coenzyme A carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. ab109368 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (<a href='/en-us/products/primary-antibodies/acetyl-coenzyme-a-carboxylase-antibody-epr4971-ab109368'>ab109368</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Hela whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 266 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded brain tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (AB231681)

This data was developed using ab109368, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (<a href='/en-us/products/primary-antibodies/acetyl-coenzyme-a-carboxylase-antibody-epr4971-ab109368'>ab109368</a>) at 1/5000 dilution

Lane 1:

293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 266 kDa

Observed band size: 266 kDa

false