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AB223781

Anti-Acetyl Coenzyme A Carboxylase beta antibody

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(1 Publication)

Rabbit Polyclonal Acetyl Coenzyme A Carboxylase beta antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACACB aa 1-200.

View Alternative Names

ACC2, ACCB, ACACB, Acetyl-CoA carboxylase 2, ACC-beta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase beta antibody (AB223781)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase beta antibody (AB223781)

Paraffin-embedded human soft tissue stained for Acetyl Coenzyme A Carboxylase beta using ab223781 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Acetyl Coenzyme A Carboxylase beta antibody (AB223781)
  • WB

Supplier Data

Western blot - Anti-Acetyl Coenzyme A Carboxylase beta antibody (AB223781)

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase beta antibody (ab223781) at 1/250 dilution

All lanes:

Human adipose tissue lysate

Predicted band size: 277 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACACB aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O00763

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase beta (ACCβ) also known as ACC2 is an enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA. This enzyme has a molecular mass of about 280 kDa. Mechanically ACCβ is important in initiating fatty acid biosynthesis. ACCβ is expressed most abundantly in skeletal muscle liver and heart tissues. These tissues have a significant demand for fatty acid metabolism and energy regulation.
Biological function summary

The enzyme controls fatty acid oxidation by regulating malonyl-CoA levels which in turn inhibits carnitine palmitoyltransferase 1 (CPT1) in the mitochondrial membrane. This action helps balance the partitioning between fatty acid synthesis and oxidation. ACCβ functions not in isolation but as part of a larger complex that can undergo polymerization or depolymerization impacting its activity. ACCβ impacts energy homeostasis by influencing cellular energy stores and metabolic flux.

Pathways

ACCβ plays a significant role in the fatty acid metabolism and insulin signaling pathways. It is a pivotal enzyme in the control of lipid biosynthesis and oxidation balance. Key proteins related to these pathways include AMP-activated protein kinase (AMPK) which phosphorylates and inactivates ACCβ therefore modulating its activity in response to energy levels. This regulation integrates nutritional signals with energy metabolism.

ACCβ is linked to metabolic disorders such as obesity and type 2 diabetes. Alterations in ACCβ function can lead to dysregulation of lipid metabolism contributing to these conditions. Also through its role in fatty acid metabolism ACCβ has connections to diseases mediated by CPT1 dysregulation potentially affecting heart disease and other lipid-associated disorders where metabolic imbalance plays a role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed : 16854592, PubMed : 19236960, PubMed : 19900410, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 19236960, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).
See full target information ACACB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40954077

2025

LncRNA-CTD suppresses metastasis and immune evasion by modulating snail1 and MHC-I expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xu,Huisi Qiu,Yuezhang Sun,Hong Jin,Siyu Yan,Fangmei Zhang,Ying Song,Guopei Zheng,Dongjiang Liao,Xiaoli Wei,Liyun Luo
View all publications
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Product promise

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