Rabbit Polyclonal Acetyl Coenzyme A Carboxylase beta antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACACB aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACACB aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O00763
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed:16854592, PubMed:19236960, PubMed:19900410, PubMed:20457939, PubMed:20952656, PubMed:26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:19236960, PubMed:20457939, PubMed:20952656, PubMed:26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).
Alternative names
ACC2, ACCB, ACACB, Acetyl-CoA carboxylase 2, ACC-beta
Recommended products
Rabbit Polyclonal Acetyl Coenzyme A Carboxylase beta antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACACB aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACACB aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O00763
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetyl Coenzyme A Carboxylase beta (ACCβ) also known as ACC2 is an enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA. This enzyme has a molecular mass of about 280 kDa. Mechanically ACCβ is important in initiating fatty acid biosynthesis. ACCβ is expressed most abundantly in skeletal muscle liver and heart tissues. These tissues have a significant demand for fatty acid metabolism and energy regulation.
- Biological function summary
The enzyme controls fatty acid oxidation by regulating malonyl-CoA levels which in turn inhibits carnitine palmitoyltransferase 1 (CPT1) in the mitochondrial membrane. This action helps balance the partitioning between fatty acid synthesis and oxidation. ACCβ functions not in isolation but as part of a larger complex that can undergo polymerization or depolymerization impacting its activity. ACCβ impacts energy homeostasis by influencing cellular energy stores and metabolic flux.
- Pathways
ACCβ plays a significant role in the fatty acid metabolism and insulin signaling pathways. It is a pivotal enzyme in the control of lipid biosynthesis and oxidation balance. Key proteins related to these pathways include AMP-activated protein kinase (AMPK) which phosphorylates and inactivates ACCβ therefore modulating its activity in response to energy levels. This regulation integrates nutritional signals with energy metabolism.
- Associated diseases and disorders
ACCβ is linked to metabolic disorders such as obesity and type 2 diabetes. Alterations in ACCβ function can lead to dysregulation of lipid metabolism contributing to these conditions. Also through its role in fatty acid metabolism ACCβ has connections to diseases mediated by CPT1 dysregulation potentially affecting heart disease and other lipid-associated disorders where metabolic imbalance plays a role.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase beta antibody (ab223781)
Paraffin-embedded human soft tissue stained for Acetyl Coenzyme A Carboxylase beta using ab223781 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Acetyl Coenzyme A Carboxylase beta antibody (ab223781)
All lanes: Western blot - Anti-Acetyl Coenzyme A Carboxylase beta antibody (ab223781) at 1/250 dilution
All lanes: Human adipose tissue lysate
Predicted band size: 277 kDa
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Product protocols
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