AB173583

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal ACACA phospho S79 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

2 Images

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (AB173583)

Lanes 1 - 3 : Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (<a href='/en-us/products/primary-antibodies/acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-ab68191'>ab68191</a>) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ACACA knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HEK293 whole cell lysate at 20 µg

Predicted band size: 266 kDa

Observed band size: 266 kDa

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Unknown

Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (AB173583)

Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

Unconjugated

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1885Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab173583 is the carrier-free version of ab68191.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.

Biological function summary

Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.

Pathways

Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.

Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443).

See full target information ACACA phospho S79

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1271 PubMed34594408

2021

TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhao,Zhenyu Wang,Yehong Chi,Chunmei Ni,Xudan Zheng

Journal of experimental & clinical cancer research : CR 37:100 PubMed29743122

2018

SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Chen,Ya-Wen Wang,Peng Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (AB173583)

Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (AB173583)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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