Rabbit Polyclonal acetyl Lysine antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Modified Amino Acid samples. Cited in 42 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to acetyl Lysine.
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- Redox biology 58:1025342022SRT1720 as an SIRT1 activator for alleviating paraquat-induced models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Chang Chao,Chuen-Lin Huang,Jing-Jy Cheng,Chun-Tang Chiou,I-Jung Lee,Ying-Chen Yang,Ting-Huang Hsu,Chia-En Yei,Pei-Ying Lin,Jih-Jung Chen,Nai-Kuei HuangPubMed 36379180
- Oncotarget 13:890-9042022CUDC907, a dual phosphoinositide-3 kinase/histone deacetylase inhibitor, promotes apoptosis of NF2 Schwannoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulianne Huegel,Christine T Dinh,Maria Martinelli,Olena Bracho,Rosa Rosario,Haley Hardin,Michael Estivill,Anthony Griswold,Sakir Gultekin,Xue-Zhong Liu,Cristina Fernandez-VallePubMed 35875610
- Cell reports 40:1110882022Viral E protein neutralizes BET protein-mediated post-entry antagonism of SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene P Chen,James E Longbotham,Sarah McMahon,Rahul K Suryawanshi,Mir M Khalid,Taha Y Taha,Takako Tabata,Jennifer M Hayashi,Frank W Soveg,Jared Carlson-Stevermer,Meghna Gupta,Meng Yao Zhang,Victor L Lam,Yang Li,Zanlin Yu,Erron W Titus,Amy Diallo,Jennifer Oki,Kevin Holden,Nevan Krogan,Danica Galonić Fujimori,Melanie OttPubMed 35839775
- Molecular therapy. Nucleic acids 27:621-6442022Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdilene Siqueira,Aida Obiols-Guardia,Olga C Jorge-Torres,Cristina Oliveira-Mateos,Marta Soler,Deepthi Ramesh-Kumar,Fernando Setién,Daniëlle van Rossum,Ainhoa Pascual-Alonso,Clara Xiol,Cristina Ivan,Masayoshi Shimizu,Judith Armstrong,George A Calin,R Jeroen Pasterkamp,Manel Esteller,Sonia GuilPubMed 35036070
- bioRxiv : the preprint server for biology :2021Viral E Protein Neutralizes BET Protein-Mediated Post-Entry Antagonism of SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene P Chen,James E Longbotham,Sarah McMahon,Rahul K Suryawanshi,Jared Carlson-Stevermer,Meghna Gupta,Meng Yao Zhang,Frank W Soveg,Jennifer M Hayashi,Taha Y Taha,Victor L Lam,Yang Li,Zanlin Yu,Erron W Titus,Amy Diallo,Jennifer Oki,Kevin Holden,Nevan Krogan,Danica Galonić Fujimori,Melanie OttPubMed 34816261
- Frontiers in chemistry 9:7472362021Acetylated Thioredoxin Reductase 1 Resists Oxidative Inactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid E Wright,Nikolaus Panaseiko,Patrick O'DonoghuePubMed 34604175
- Redox biology 46:1020382021Mechanisms of impaired mitochondrial homeostasis and NAD metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShannon Chiang,Nady Braidy,Sanaz Maleki,Sean Lal,Des R Richardson,Michael L-H HuangPubMed 34416478
- International journal of molecular sciences 22:2021Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlsu Z Malikova,Anastasia S Shcherbakova,Konstantin A Konduktorov,Anastasia S Zemskaya,Alexandra A Dalina,Vladimir I Popenko,Olga G Leonova,Alexei V Morozov,Nikolay N Kurochkin,Olga A Smirnova,Sergey N Kochetkov,Maxim V KozlovPubMed 33925399
- Journal of biochemical and molecular toxicology 35:1-142021Ameliorative effect of betanin on experimental cisplatin-induced liver injury; the novel impact of miRNA-34a on the SIRT1/PGC-1α signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsmail El Shaffei,Ghada A Abdel-Latif,Doaa B Farag,Mona Schaalan,Rania M SalamaPubMed 33661552
- Scientific reports 11:39152021The miRNA199a/SIRT1/P300/Yy1/sST2 signaling axis regulates adverse cardiac remodeling following MI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Carmen Asensio-Lopez,Yassine Sassi,Fernando Soler,Maria Josefa Fernandez Del Palacio,Domingo Pascual-Figal,Antonio LaxPubMed 33594087
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to acetyl Lysine.
Consider this alternative
Reactivity data
Select an application| IP | ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Modified Amino Acid | Expected | Expected | Tested | Tested | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes (see Abreview) |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Amino Acid | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Alternative names
pan acetyl Lysine
Recommended products
Rabbit Polyclonal acetyl Lysine antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Modified Amino Acid samples. Cited in 42 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to acetyl Lysine.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to acetyl Lysine.
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetyl lysine often referred to as acetylated lysine is a modification of the amino acid lysine where an acetyl group is transferred to the lysine residue. This modification impacts the mass of the lysine residue slightly altering its properties. Acetyl lysine occurs in histone proteins and this modification is an important regulator of chromatin structure and function. The acetylation of lysine residues happens frequently in the nucleus of eukaryotic cells where histone acetylases (HATs) facilitate this process. Known alternatively as lysine acetylation it represents an important post-translational modification impacting gene expression regulation.
- Biological function summary
Acetylation adds an acetyl group to the lysine which changes its positive charge reducing chromatin compaction and enhancing gene transcription. The acetyl lysine modification is not acting alone; it forms part of larger histone modification complexes. By altering chromatin structure acetyl lysine regulates the accessibility of transcription factors to DNA influencing various biological processes like DNA repair replication and cell cycle progression. The interaction between acetyl lysine and chromatin remodeling complexes plays a significant role in epigenetic regulation.
- Pathways
Acetyl lysine is central to epigenetic regulatory pathways significantly impacting gene expression and cellular growth pathways. The acetylation process involves histone acetylases (HATs) such as the p300/CBP complex which adds acetyl groups to specific lysines on histones increasing transcriptional activity. Conversely histone deacetylases (HDACs) remove acetyl groups decreasing transcription. The balance of these modifications involves critical pathways such as the TGF-beta signaling pathway and the NF-kB pathway linking acetyl lysine with numerous cellular activities and protein interactions.
- Associated diseases and disorders
Dysregulation of acetyl lysine levels is associated with cancer and neurodegenerative diseases. In cancer the aberrant acetylation of lysine impacts expression of oncogenes and tumor suppressor genes contributing to carcinogenesis. Proteins like p53 which relies on acetylation for activation become functionally impaired. Also in neurodegenerative disorders such as Alzheimer's disease histone acetylation imbalances disrupt neuronal function and gene expression. The involvement of the sirtuin family of proteins which act as NAD+-dependent deacetylases connects lysine acetylation to these diseases highlighting its importance in maintaining normal cellular functions and its role in pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (ab80178)
Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A FITC conjugated Goat Anti-Rabbit secondary antibody (green) was used at 1/200 dilution. Localization: Nucleus and Cytoplasm.
(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.
Image courtesy of Dr Natasha Snider by customer review.Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (ab80178)
ab80178 staining acetyl Lysine in human HepG2 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 2% serum for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/150 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (ab80178)
Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A R-PE conjugated Goat Anti-Rabbit secondary antibody (yellow) was used at 1/200 dilution. Localization: Nucleus and Cytoplasm.
(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.
Image courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (ab80178)
ab80178 staining acetyl Lysine (green) in the neuromuscular junction of fruit fly cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in formaldehde, permeabilized using 0.4% Triton-X, blocked with 10% NGS for 1 hour at 20°C, then incubated with ab80178 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.
Western blot - Anti-acetyl Lysine antibody (ab80178)
Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Primary Antibody: Rabbit Anti-Acetyl Lysine Polyclonal Antibody (ab80178) at 1:1000.
All lanes: Western blot - Anti-acetyl Lysine antibody (ab80178)
All lanes: Cell lysates prepared from TSA treated mouse spleen cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acetyl Lysine antibody (ab80178)
ab80178 staining acetyl Lysine in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.
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