Anti-acetyl Lysine antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (AB80178)

Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A FITC conjugated Goat Anti-Rabbit secondary antibody (green) was used at 1/200 dilution. Localization : Nucleus and Cytoplasm.

(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (AB80178)

Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A R-PE conjugated Goat Anti-Rabbit secondary antibody (yellow) was used at 1/200 dilution. Localization : Nucleus and Cytoplasm.

(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.

• IHC-P

AbReview24393****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acetyl Lysine antibody (AB80178)

ab80178 staining acetyl Lysine in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

• ICC/IF

AbReview23512****

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (AB80178)

ab80178 staining acetyl Lysine in human HepG2 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 2% serum for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/150 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.

Image courtesy of Dr Natasha Snider by Abreview.

• ICC/IF

AbReview26148****

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (AB80178)

ab80178 staining acetyl Lysine (green) in the neuromuscular junction of fruit fly cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in formaldehde, permeabilized using 0.4% Triton-X, blocked with 10% NGS for 1 hour at 20°C, then incubated with ab80178 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Anti-acetyl Lysine antibody (AB80178)

Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Primary Antibody : Rabbit Anti-Acetyl Lysine Polyclonal Antibody (ab80178) at 1 : 1000.

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Western blot - Anti-acetyl Lysine antibody (ab80178)

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Cell lysates prepared from TSA treated mouse spleen cells

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