Goat Polyclonal Acetylcholinesterase antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human ACHE aa 600 to C-terminus.
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- Gut :2022Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina F Ruivo,Nuno Bastos,Barbara Adem,Ines Batista,Cecilia Duraes,Carlos A Melo,Stephanie A Castaldo,Francisco Campos-Laborie,Pedro Moutinho-Ribeiro,Barbara Morão,Ana Costa-Pinto,Soraia Silva,Hugo Osorio,Sergio Ciordia,Jose Luis Costa,David Goodrich,Bruno Cavadas,Luisa Pereira,Tony Kouzarides,Guilherme Macedo,Rui Maio,Fatima Carneiro,Marília Cravo,Raghu Kalluri,Jose Carlos Machado,Sonia A MeloPubMed 35012996
- The EMBO journal 40:e1054922021Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorena Martin-Jaular,Nathalie Nevo,Julia P Schessner,Mercedes Tkach,Mabel Jouve,Florent Dingli,Damarys Loew,Kenneth W Witwer,Matias Ostrowski,Georg H H Borner,Clotilde ThéryPubMed 33709510
- Journal of extracellular vesicles 10:e120502020Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFederico Cocozza et. al.PubMed 33391636
- FEBS open bio 10:2805-28232020Interaction of amyloid beta with humanin and acetylcholinesterase is modulated by ATP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Atali et. al.PubMed 33145964
- International journal of biological macromolecules 160:35-462020The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReza Yekta et. al.PubMed 32454110
- Biochemistry 59:1981-20022020Humanin Blocks the Aggregation of Amyloid-β Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeanna Price et. al.PubMed 32383868
- Scientific reports 10:50832020IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeanna Price et. al.PubMed 32193421
- Journal of extracellular vesicles 8:16285922019Acetylcholinesterase is not a generic marker of extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaohao Liao et. al.PubMed 31303981
- Journal of Alzheimer's disease : JAD 53:831-412016Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria-Letizia Campanari,Francisco Navarrete,Stephen D Ginsberg,Jorge Manzanares,Javier Sáez-Valero,María-Salud García-AyllónPubMed 27258420
- PloS one 9:e1134212014Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain.Applications:WBReactive species:HumanRyan P Vetreno,Margaret Broadwater,Wen Liu,Linda P Spear,Fulton T CrewsPubMed 25405505
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ACHE aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22303
Reactivity data
Select an application| WB | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Cat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Cat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Cat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Cow, Cat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Alternative names
Acetylcholinesterase, AChE, ACHE
Recommended products
Goat Polyclonal Acetylcholinesterase antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human ACHE aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ACHE aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22303
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.
- Biological function summary
Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.
- Pathways
Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.
- Associated diseases and disorders
Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Acetylcholinesterase antibody (ab31276)
Detected by chemiluminescence.
All lanes: Western blot - Anti-Acetylcholinesterase antibody (ab31276) at 0.3 µg/mL
Lane 1: Jurkat cell lysate at 0.5 µg/mL
Lane 2: HepG2 cell lysate in RIPA buffer at 35 µg
Predicted band size: 68 kDa
This image is courtesy of a customer review submitted by Ender AvciWestern blot - Anti-Acetylcholinesterase antibody (ab31276)
All lanes: Western blot - Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1: SkHep1 cell lysate at 50 µg
Lane 2: Snu387 cell lysate at 50 µg
Lane 3: SW620 cell lysate at 50 µg
Lane 4: Zebrafish brain lysate at 50 µg
SecondaryAll lanes: HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20min
Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (ab31276)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry - Anti-Acetylcholinesterase antibody (ab31276)
Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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