AB97299

Anti-Acetylcholinesterase antibody

Name: Anti-Acetylcholinesterase antibody (ab97299) | Abcam
Brand: Abcam Limited
SKU: AB97299

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(5 Publications)

Rabbit Polyclonal Acetylcholinesterase antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACHE aa 400-650.

View Alternative Names

Acetylcholinesterase, AChE, ACHE

10 Images

Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (AB97299)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (AB97299)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97299 (1) and merged with DNA probe (2) at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Paraffin embedded rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Paraffin embedded mouse colon tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody (AB97299)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody (AB97299)

Frozen sectioned E13.5 rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Paraffin embedded rat fore brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Paraffin embedded mouse brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Acetylcholinesterase antibody (ab97299) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

All lanes:

Western blot - Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution

Lane 1:

Mouse Brain lysate at 50 µg

Lane 2:

Rat brain lysate at 50 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

All lanes:

Western blot - Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution

All lanes:

Rat brain at 50 µg

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

All lanes:

Western blot - Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution

Lane 1:

Mouse brain at 50 µg

Lane 2:

Mouse lung at 50 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human ACHE aa 400-650. The exact immunogen used to generate this antibody is proprietary information.

P22303

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.

Biological function summary

Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.

Pathways

Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.

Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.

See full target information ACHE

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 24:100960 PubMed40180179

2025

Multi-Omics Analysis Unveils Nsun5-Mediated Molecular Alterations in the Somatosensory Cortex and Its Impact on Pain Sensation.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Chen,Heyu Zhao,Xia Gao,Junchao Xu,Zhili Huang,Huali Shen

Frontiers in surgery 11:1407948 PubMed39315293

2024

Paneth-like cells disruption and intestinal dysbiosis in the development of enterocolitis in an iatrogenic rectosigmoid hypoganglionosis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Iskandar Rahardjo Budianto,Kusmardi Kusmardi,Andi Muh Maulana,Somasundaram Arumugam,Rejina Afrin,Vivian Soetikno

Journal of clinical medicine 11: PubMed35743382

2022

Effects of Smart Drugs on Cholinergic System and Non-Neuronal Acetylcholine in the Mouse Hippocampus: Histopathological Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Ryusei Satoh,Kiyoharu Kawakami,Kazuhiko Nakadate

Brain pathology (Zurich, Switzerland) 29:502-512 PubMed30511454

2019

Early growth response-1 regulates acetylcholinesterase and its relation with the course of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ting Hu,Xin-Lu Chen,Shu-Han Huang,Qiong-Bin Zhu,Si-Yang Yu,Yi Shen,Arja Sluiter,Joost Verhaagen,Juan Zhao,Dick Swaab,Ai-Min Bao

Shock (Augusta, Ga.) 47:175-183 PubMed27648692

2016

Burn Injury Alters Epidermal Cholinergic Mediators and Increases HMGB1 and Caspase 3 in Autologous Donor Skin and Burn Margin.

Applications

Unspecified application

Species

Unspecified reactive species

Casey J Holmes,Jennifer K Plichta,Richard L Gamelli,Katherine A Radek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Acetylcholinesterase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (AB97299)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody (AB97299)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (AB97299)

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

Western blot - Anti-Acetylcholinesterase antibody (AB97299)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise