Rabbit Recombinant Monoclonal Acetylcholinesterase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 13 publications.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
WB | IHC-Fr | IHC-P | |
---|---|---|---|
Mouse | Tested | Tested | Tested |
Rat | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info 1/1000 | Notes Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Acetylcholinesterase, AChE, Ache
Rabbit Recombinant Monoclonal Acetylcholinesterase antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 13 publications.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Acetylcholinesterase also known as AChE is an enzyme with a molecular mass of approximately 67 kDa. It plays a critical role in neurotransmission by catalyzing the hydrolysis of the neurotransmitter acetylcholine into acetate and choline. This reaction occurs at neuromuscular junctions and cholinergic synapses therefore terminating synaptic transmission. AChE is highly expressed in muscle and brain tissue particularly in the synaptic cleft where it regulates the nerve signal terminations.
Acetylcholinesterase is essential for maintaining neurotransmission dynamics by ensuring timely acetylcholine breakdown. It does not function as part of a larger enzyme complex but its activity is necessary for efficient synaptic signaling in the nervous system. This enzymatic action prevents continuous stimulation of muscles and nerves by rapidly degrading acetylcholine thereby ensuring proper muscle contraction and cognitive processes.
Acetylcholinesterase participates significantly in the cholinergic system. It influences cholinergic signaling pathways by inactivating acetylcholine after its release into the synaptic cleft. This function aligns acetylcholinesterase closely with receptors like nicotinic and muscarinic acetylcholine receptors. It indirectly affects signal transduction pathways that involve these receptors with potential downstream effects on ion channels and intracellular messengers.
Acetylcholinesterase plays a significant role in Alzheimer's disease and myasthenia gravis. In Alzheimer's disease decreased acetylcholinesterase function can lead to accumulations of acetylcholine and disrupted signaling contributing to cognitive dysfunction. Acetylcholinesterase inhibitors are therapeutic in such contexts. For myasthenia gravis a disorder affecting neuromuscular transmission the enzyme’s interaction with antibodies targets synaptic acetylcholine receptors. This interaction results in weakened muscle contractions correlating with condition severity.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 30 seconds, Lane 2: 3 minutes.
Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
All lanes: Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution
Lane 1: Mouse brain lysate at 20 µg
Lane 2: Mouse striatum lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
All lanes: Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution
Lane 1: Rat striatum lysate at 20 µg
Lane 2: Rat hippocampus lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 3min
Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Acetylcholinesterase western blot using anti-Acetylcholinesterase antibody [EPR18978] ab183591. Publication image and figure legend from Liu, B., Kou, J., et al., 2020, Aging (Albany NY), PubMed 32392535.
ab183591 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183591 please see the product overview.
Effects of LEO on acetylcholinesterase content in APP/PS1 mice and WT mice. Hippocampus of each group was extracted by extracting buffer and then estimated by Western blot. (A) Representative Western blot of acetylcholinesterase. (B) Densitometric analyses of the immunoreactivity to the antibody shown in A. (C) Quantitative analysis for the relative intensity of AChE in hippocampus. (D) Immunostaining with anti-AChE antibody in each group. Scale bar, hippocampus = 100 μm, CA1, CA3 = 20 μm, DG = 50 μm respectively. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, significantly different from non-treated mice group. LEO: lemon essential oil.
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