Anti-Acetylcholinesterase antibody [EPR18978]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Blocking/Dilution buffer : 5% NFDM/TBST.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID : 2400605; PMID 8515842).

All lanes:

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1:

Rat striatum lysate at 20 µg

Lane 2:

Rat hippocampus lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 30 seconds, Lane 2 : 3 minutes.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID : 2400605; PMID 8515842).

All lanes:

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse striatum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

• WB

CiteAb

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (AB183591)

Acetylcholinesterase western blot using anti-Acetylcholinesterase antibody [EPR18978] ab183591. Publication image and figure legend from Liu, B., Kou, J., et al., 2020, Aging (Albany NY), PubMed 32392535.

ab183591 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183591 please see the product overview.

Effects of LEO on acetylcholinesterase content in APP/PS1 mice and WT mice. Hippocampus of each group was extracted by extracting buffer and then estimated by Western blot. (A) Representative Western blot of acetylcholinesterase. (B) Densitometric analyses of the immunoreactivity to the antibody shown in A. (C) Quantitative analysis for the relative intensity of AChE in hippocampus. (D) Immunostaining with anti-AChE antibody in each group. Scale bar, hippocampus = 100 μm, CA1, CA3 = 20 μm, DG = 50 μm respectively. Data are expressed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, significantly different from non-treated mice group. LEO : lemon essential oil.

false