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AB166820

Anti-Achaete-scute homolog 3 antibody

4

(1 Review)

|

(2 Publications)

Goat Polyclonal Achaete-scute homolog 3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ASCL3 aa 100-150.

View Alternative Names

BHLHA42, HASH3, SGN1, ASCL3, Achaete-scute homolog 3, ASH-3, hASH3, Class A basic helix-loop-helix protein 42, bHLH transcriptional regulator Sgn-1, bHLHa42

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Achaete-scute homolog 3 antibody (AB166820)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Achaete-scute homolog 3 antibody (AB166820)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain (cortex) tissue labeling Achaete-scute homolog 3 with ab166820 at 3.75 µg/ml.

Western blot - Anti-Achaete-scute homolog 3 antibody (AB166820)
  • WB

Unknown

Western blot - Anti-Achaete-scute homolog 3 antibody (AB166820)

Incubation with ab166820 was 1 hour.

All lanes:

Western blot - Anti-Achaete-scute homolog 3 antibody (ab166820) at 0.1 µg/mL

All lanes:

Human liver lysate (in RIPA buffer) at 35 µg

Predicted band size: 20 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ASCL3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ33

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Achaete-scute homolog 3 also known as ASCL3 is a transcription factor with a mass of approximately 24 kDa. This protein belongs to the basic helix-loop-helix (bHLH) family and plays a role in the regulation of neuronal differentiation. ASCL3 shows expression predominantly in neurogenic regions including areas within the brain and peripheral nervous system. It functions by binding to E-box DNA sequences which promotes the expression of genes involved in neurogenesis.
Biological function summary

Achaete-scute homolog 3 drives the differentiation of neuronal precursor cells into mature neurons. It is part of the proneural gene network where it interacts with other bHLH transcription factors to regulate the transcriptional cascade necessary for neuronal development. By forming heterodimeric complexes with E-proteins ASCL3 enhances transcriptional activity thereby promoting the expression of genes critical for neuronal identity and function.

Pathways

Achaete-scute homolog 3 plays a significant role in the Notch signaling pathway which is important for maintaining the balance between neuronal progenitor proliferation and differentiation. It interacts with proteins such as HES1 and HES5 which are also involved in the Notch pathway. By influencing the expression of these pathway components ASCL3 ensures the proper development of neural structures and maintains the equilibrium between different cell fates in neurogenic tissues.

Achaete-scute homolog 3 has been associated with neurodevelopmental disorders. Alterations in its expression or function may contribute to conditions such as schizophrenia and autism spectrum disorders. ASCL3 is connected to other proteins in these conditions such as the DISC1 protein in schizophrenia. Understanding how ASCL3 interacts with these proteins can offer insights into the molecular mechanisms underlying these neurological conditions and potentially guide therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor. Inhibits myogenesis. Plays a role in progenitor cells which differentiate into ductal and acinar, but not myoepithelial, cell lineages in the salivary glands. Involved in the functions of the microvillar cells and Bowman's glands and probably, in a non-cell-autonomous manner, in the development or regeneration of a complete olfactory epithelium (OE).
See full target information ASCL3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 11:127 PubMed32197647

2020

Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Sui,Siqi Zhang,Yongliang Li,Xin Zhang,Waner Hu,Yanrui Feng,Jingwei Xiong,Yuanyuan Zhang,Shicheng Wei

Journal of molecular cell biology 7:476-85 PubMed26260029

2015

Nucleoporin 35 regulates cardiomyocyte pH homeostasis by controlling Na+-H+ exchanger-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Lei Pan,Jun Li,Bijun Huang,Jing Feng,Changming Li,Shiyi Wang,Erlinda The,Yuan Liu,Tianyou Yuan,Lixiao Zhen,Dandan Liang,Yi Liu,Li Li,Yingyu Cui,Xiaoyan Jiang,Luying Peng,Yi-Han Chen
View all publications
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