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AB249379

Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPAC antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1

4 Images
Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)
  • WB

Lab

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)

This data was developed using ab166896, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab166896 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-acp1-antibody-epr9839-ab166896'>ab166896</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)
  • WB

Unknown

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)

This data was developed using ab166896, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-acp1-antibody-epr9839-ab166896'>ab166896</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 18 kDa

false

Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)
  • IP

Unknown

Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)

This data was developed using ab166896, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-acp1-antibody-epr9839-ab166896'>ab166896</a>)

Predicted band size: 18 kDa

false

Flow Cytometry (Intracellular) - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Acid phosphatase/ACP1 antibody [EPR9839] - BSA and Azide free (AB249379)

This data was developed using ab166896, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase/ACP1 using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).

  • Unconjugated

    Anti-Acid phosphatase/ACP1 antibody [EPR9839]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9839

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab249379 is the carrier-free version of ab166896.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary

The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.

Pathways

The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.

Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.. Isoform 3. Does not possess phosphatase activity.
See full target information ACP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com