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Rabbit Recombinant Monoclonal PPAC antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Acid phosphatase antibody [EPR21791] (AB235449), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] (AB235449), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] (AB235449), expandable thumbnail
  • Western blot - Anti-Acid phosphatase antibody [EPR21791] (AB235449), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] (AB235449), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/600
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2. Isoform 3. Does not possess phosphatase activity.

Alternative names

Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1

Recommended products

Rabbit Recombinant Monoclonal PPAC antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21791
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Acid phosphatase also known as tartrate-resistant acid phosphatase (TRAP) or prostatic acid phosphatase (PAP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an acidic environment. It has a molecular mass of approximately 50 kDa and is expressed in various human tissues including the prostate bone liver and spleen. Within the prostate it breaks down and releases phosphate from different compounds and it plays a role in bone resorption by osteoclasts.

Biological function summary

This enzyme contributes to multiple cellular processes including the regulation of bone metabolism and prostate health. Acid phosphatase when acting as TRAP is often found within osteoclast lysosomes where it degrades bone matrix components. It does not form part of a complex but functions independently to modulate these processes. Prostatic acid phosphatase in seminal fluid has additional roles particularly in prostate tissue affecting cellular proliferation and motility.

Pathways

Acid phosphatase participates in both bone resorption and lysosomal degradation pathways. In bone metabolism it works alongside proteins such as cathepsin K assisting in the breakdown of matrix constituents during bone remodeling. In lysosomal pathways it complements activities of enzymes like lysosomal acid lipase helping in recycling cellular materials. These interactions underline its involvement in maintaining cellular homeostasis through these critical biological pathways.

Associated diseases and disorders

Acid phosphatase relates significantly to prostate cancer and bone disorders like osteoporosis. Elevated levels of prostatic acid phosphatase in serum serve as a marker for prostate cancer linking it to androgen receptor pathways critical in disease progression. In bone disorders TRAP's role in osteoclast function connects it to conditions such as osteoporosis where its interaction with integrin receptor influences bone density regulation. Understanding these connections can aid in developing diagnostic and therapeutic strategies for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Lanes 1 - 4: Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout sample. Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab235449 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (Human ACP1 (Acid phosphatase) knockout HEK-293 cell line ab261859)

    Lane 3: K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 18 kDa

    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human colon cancer (PMID: 25811796) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa-Fluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 minutes.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 25811796).

    All lanes: Western blot - Anti-Acid phosphatase antibody [EPR21791] (ab235449) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 5: Human placenta tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  • Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanolpermeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cell line labeling Acid Phosphatase with ab235449 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Black) isotype control, and an unlabeled control (Cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa-Fluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HepG2 cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] (ab235449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] (ab235449)

    Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human prostatic hyperplasia (PMID: 26159288) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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