Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cell line labeling Acid Phosphatase with ab235449 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control, and an unlabeled control (Cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HepG2 cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human prostatic hyperplasia (PMID : 26159288) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human colon cancer (PMID : 25811796) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab235449).

• WB

Lab

Western blot - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

This data was developed using the same antibody clone in a different buffer formulation (ab235449).

Lanes 1 - 4 : Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout sample. Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab235449 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase antibody [EPR21791] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-antibody-epr21791-ab235449'>ab235449</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell lysate (<a href='/en-us/products/cell-lysates/human-acp1-acid-phosphatase-knockout-hek-293-cell-lysate-ab261668'>ab261668</a>) at 20 µg

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

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