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AB273656

Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free

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Mouse Monoclonal ASM antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMPD1 aa 50-350.

View Alternative Names

ASM, SMPD1, Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase

22 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human colon adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human endometrium tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human liver tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human liver carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human kidney tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human lung carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human kidney carcinoma tissue stained for Acid sphingomyelinase aab273656 at 1/150 diution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human colon tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human ovary tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human prostate carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human prostate tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human thyroid carcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human pancreas tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human lymph node tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human tonsil tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human lung tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human bladder tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human ovary adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human breast adenocarcinoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Paraffin-embedded human lymphoma tissue stained for Acid sphingomyelinase using ab273656 at 1/150 dilution.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris, pH 8.5, 120°C for 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Western blot - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)
  • WB

Supplier Data

Western blot - Anti-Acid sphingomyelinase antibody [OTI3H7] - BSA and Azide free (AB273656)

Cells were transfected with cDNA for 48 hrs and lysed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

All lanes:

Same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY Acid sphingomyelinase cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3H7

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SMPD1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P17405

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3 minutes.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.3 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid sphingomyelinase (ASMase) also known as sphingomyelin phosphodiesterase 1 or NP is an enzyme involved in sphingolipid metabolism. ASMase has a mass of approximately 75 kDa and appears in lysosomes where it converts sphingomyelin to ceramide and phosphorylcholine. This enzyme is important in maintaining cellular lipid balance and signaling. Expression of ASMase occurs in various tissues such as the liver spleen and brain.
Biological function summary

ASMase plays a role in membrane microdomain composition through its involvement in ceramide production. It participates in generating ceramide-enriched platforms that facilitate the clustering of signaling molecules. Ceramide acts as a second messenger in multiple cellular processes including apoptosis proliferation and inflammation. ASMase operates in the lysosomal lipid degradation pathway and connects with other lysosomal enzymes to modulate lipid turnovers such as glucosylceramidase affecting downstream cellular functions.

Pathways

Sphingolipid metabolism involves ASMase. This enzyme participates in the ceramide signaling pathway influencing apoptosis and stress responses. Related proteins in this pathway include casein kinase II which phosphorylates ASMase and cathepsin D involved in the lysosomal degradation process. ASMase activity alters ceramide levels impacting pro-apoptotic and pro-survival signals mediated by related proteins in the cell signaling network.

ASMase deficiency connects to Niemann-Pick disease types A and B characterized by lipid accumulation in lysosomes. Mutations in the ASMase gene lead to impaired enzyme function resulting in excessive sphingomyelin storage and cell damage. The disorder links ASMase to proteins such as hexa-beta-N-acetylglucosaminidase which is affected in other lysosomal storage disorders. Research shows that ASMase activity also influences cardiovascular diseases by regulating ceramide and cholesterol levels in atherosclerotic lesions connecting it to inflammatory pathways involving adhesion molecules and cytokines.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts sphingomyelin to ceramide (PubMed : 12563314, PubMed : 1840600, PubMed : 18815062, PubMed : 25339683, PubMed : 25920558, PubMed : 27659707, PubMed : 33163980). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed : 20807762, PubMed : 21098024, PubMed : 9660788). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed : 12563314, PubMed : 20530211, PubMed : 20807762, PubMed : 22573858, PubMed : 9393854).. In the lysosomes, converts sphingomyelin to ceramide (PubMed : 20807762, PubMed : 21098024). Plays an important role in the export of cholesterol from the intraendolysosomal membranes (PubMed : 25339683). Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol (PubMed : 25339683). Modulates stress-induced apoptosis through the production of ceramide (PubMed : 8706124).. When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide (PubMed : 12563314, PubMed : 17303575, PubMed : 20807762). Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses (PubMed : 12563314, PubMed : 20807762, PubMed : 9393854). Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense (PubMed : 12563314, PubMed : 20807762, PubMed : 9393854). Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P. aeruginosa, induce apoptosis and regulate the cytokine response in infected cells (PubMed : 12563314). In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair (PubMed : 20530211).. Sphingomyelin phosphodiesterase, processed form. This form is generated following cleavage by CASP7 in the extracellular milieu in response to bacterial infection (PubMed : 21157428). It shows increased ability to convert sphingomyelin to ceramide and promotes plasma membrane repair (By similarity). Plasma membrane repair by ceramide counteracts the action of gasdermin-D (GSDMD) perforin (PRF1) pores that are formed in response to bacterial infection (By similarity).. (Microbial infection) Secretion is activated by bacteria such as P. aeruginos, N. gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.. (Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.. Isoform 2. Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.. Isoform 3. Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.
See full target information SMPD1
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