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AB187381

Anti-Acidic Cytokeratin antibody [AE-1]

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(1 Publication)

Mouse Monoclonal Cytokeratin 10 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT10.

View Alternative Names

KPP, KRT10, Cytokeratin-10, Keratin-10, CK-10, K10, Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19, Cytokeratin-14, Keratin-14, CK-14, K14, KRT14, KRTB, KRT15, Cytokeratin-15, Keratin-15, CK-15, K15

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acidic Cytokeratin antibody [AE-1] (AB187381)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acidic Cytokeratin antibody [AE-1] (AB187381)

Immunohistochemistry formalin-fixed paraffin-embedded human skin tissue labeling Cytokeratin with ab187381 at 1μg/ml.

Western blot - Anti-Acidic Cytokeratin antibody [AE-1] (AB187381)
  • WB

Supplier Data

Western blot - Anti-Acidic Cytokeratin antibody [AE-1] (AB187381)

All lanes:

Western blot - Anti-Acidic Cytokeratin antibody [AE-1] (ab187381) at 1 µg/mL

All lanes:

A431 lysate

Predicted band size: 66 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT10.

P13645

Specificity

ab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.

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Complementary Products

Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

  • 5
  • 27
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 11
Primary Antibodies

AB85679

Anti-Loricrin antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody (AB85679)

  • 5
  • 11
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acidic cytokeratins often referred to as type I keratins form an essential component of the intermediate filament family of proteins. They have a molecular mass typically around 40 to 67 kDa and are found in epithelial cells. These proteins are known for their structural roles providing mechanical support and resilience to cells. Acidic cytokeratins pair with basic cytokeratins to create the cytoskeletal framework that maintains cell integrity and shape.
Biological function summary

The family of acidic cytokeratins contributes significantly to cell differentiation migration and signal transduction. They often form complexes with basic keratins which are critical for the stability and function of keratin networks. In various epithelial tissues these complexes protect cells against mechanical stress and participate in dynamic cellular processes like wound healing and epithelial repair.

Pathways

Acidic cytokeratins participate in cellular pathways related to the cytoskeleton and cell adhesion such as the Wnt and MAPK signaling pathways. These pathways influence epithelial cell behavior and interaction with their microenvironment. Related proteins in these pathways include beta-catenin which interacts with cytoskeletal components during signal transduction and cell junction remodeling.

Irregular expression of acidic cytokeratins associates with several cancers including breast and colorectal cancer. These proteins can serve as biomarkers for tumor diagnosis and progression. Additionally mutations in acidic cytokeratins link to skin diseases like epidermolysis bullosa simplex a condition disrupting normal skin structure. Proteins such as p53 which are involved in cell cycle regulation may also connect with acidic cytokeratins in the context of these diseases highlighting their significance in diagnostic and therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity).. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
See full target information KRT10

Additional targets

,KRT15,Keratin, type I cytoskeletal 14,KRT19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 40:569-575 PubMed28656288

2017

Vascular endothelial growth inhibitor 174 and its functional domains inhibit epithelial-mesenchymal transition in renal cell carcinoma cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zhang,Baoan Hong,Wenyong Lian,Changhua Zhou,Siqi Chen,Xin Du,Xiaohu Deng,Shayiremu Duoerkun,Qing Li,Yong Yang,Kan Gong
View all publications
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Product promise

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