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AB74091

Anti-ACK1 (phospho Y284) antibody

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(7 Publications)

Rabbit Polyclonal ACK1 phospho Y284 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TNK2 phospho Y284.

View Alternative Names

ACK1, TNK2, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ACK1 (phospho Y284) antibody (AB74091)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ACK1 (phospho Y284) antibody (AB74091)

ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 (phospho Y284) antibody (AB74091)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 (phospho Y284) antibody (AB74091)

ab74091 at 1/50 dilution staining ACK1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Western blot - Anti-ACK1 (phospho Y284) antibody (AB74091)
  • WB

Unknown

Western blot - Anti-ACK1 (phospho Y284) antibody (AB74091)

All lanes:

Western blot - Anti-ACK1 (phospho Y284) antibody (ab74091) at 1/500 dilution

Lane 1:

HepG2 cell extractstreated with EGF (200ng/ml, 30mins) at 30 µg

Lane 2:

HepG2 cell extractstreated with EGF (200ng/ml, 30mins) at 30 µg with immunising peptide

Predicted band size: 115 kDa

Observed band size: >117 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TNK2 phospho Y284. The exact immunogen used to generate this antibody is proprietary information.

Q07912

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab74091 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACK1 also known as TNK2 is a non-receptor tyrosine kinase with a molecular mass of approximately 120 kDa. It primarily localizes in the cytoplasm but can also translocate to the membrane and nucleus. This protein is ubiquitously expressed with notable presence in tissues like the brain liver and muscles. ACK1 plays a significant role in cellular signaling by phosphorylating various substrates impacting processes like cell proliferation migration and survival.
Biological function summary

The functions of ACK1 relate to signal transduction processes. It acts as an important part of the signaling complex involved in cell growth and differentiation. ACK1 interacts with several growth factor receptors and coordinates signal transduction events. These interactions allow ACK1 to participate in complex networks that regulate cellular shaping and organization making it important for normal cell functionality.

Pathways

ACK1 significantly influences cellular processes through the MAPK and PI3K pathways. These pathways are essential for cell survival proliferation and metabolism. ACK1 interacts with proteins such as ERK and AKT mediating downstream signaling that determines cellular responses to external stimuli. The kinase activity of ACK1 regulates various signaling cascades emphasizing its role in controlling metabolic and growth signals.

ACK1 shows relevance to cancer and neurodegenerative diseases. In cancer ACK1 overexpression associates with increased tumor growth and metastasis linked with proteins like HER2 in breast cancer. In neurodegenerative diseases abnormal ACK1 activity aligns with the progression of conditions like Alzheimer's disease where it interacts with tau proteins. Understanding ACK1's involvement in these diseases highlights its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP (PubMed : 20110370).
See full target information TNK2 phospho Y284
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 29:3151-3161 PubMed37363966

2023

Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Caner Saygin,Giorgia Giordano,Kathryn Shimamoto,Bart Eisfelder,Anika Thomas-Toth,Girish Venkataraman,Vijayalakshmi Ananthanarayanan,Tiffaney L Vincent,Adam DuVall,Anand A Patel,Yi Chen,Fenlai Tan,Stephen P Anthony,Yu Chen,Yue Shen,Olatoyosi Odenike,David T Teachey,Barbara L Kee,James LaBelle,Wendy Stock

Molecular cancer 19:90 PubMed32404161

2020

Discovery of a novel third-generation EGFR inhibitor and identification of a potential combination strategy to overcome resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Rong Qu,Shingpan Chan,Mengzhen Lai,Linjiang Tong,Fang Feng,Hongyu Chen,Tingting Song,Peiran Song,Gang Bai,Yingqiang Liu,Yanan Wang,Yan Li,Yi Su,Yanyan Shen,Yiming Sun,Yi Chen,Meiyu Geng,Ke Ding,Jian Ding,Hua Xie

Developmental dynamics : an official publication of the American Association of Anatomists 248:795-812 PubMed31219639

2019

Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina R Fritz,Yanping Zhang,L Bruno Ruest

Medicine 98:e15232 PubMed31008954

2019

The clinicopathological significance of ubiquitin-conjugating enzyme E2C, leucine-rich repeated-containing G protein-coupled receptor, WW domain-containing oxidoreductase, and vasculogenic mimicry in invasive breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Shen,Ting Wu,Pan Huang,Qixiang Shao,Miao Chen

Cancer research 76:127-38 PubMed26677978

2015

Identification and Characterization of Tyrosine Kinase Nonreceptor 2 Mutations in Leukemia through Integration of Kinase Inhibitor Screening and Genomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia E Maxson,Melissa L Abel,Jinhua Wang,Xianming Deng,Sina Reckel,Samuel B Luty,Huahang Sun,Julie Gorenstein,Seamus B Hughes,Daniel Bottomly,Beth Wilmot,Shannon K McWeeney,Jerald Radich,Oliver Hantschel,Richard E Middleton,Nathanael S Gray,Brian J Druker,Jeffrey W Tyner

International journal of oncology 46:2057-66 PubMed25738261

2015

ACK1 promotes hepatocellular carcinoma progression via downregulating WWOX and activating AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Binhui Xie,Qinshan Zen,Xiaonong Wang,Xiao He,Yuankang Xie,Zixiang Zhang,Heping Li

EMBO reports 15:1184-91 PubMed25223282

2014

Ack kinase regulates CTP synthase filaments during Drosophila oogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Todd I Strochlic,Kevin P Stavrides,Sam V Thomas,Emmanuelle Nicolas,Alana M O'Reilly,Jeffrey R Peterson
View all publications
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