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AB236773

Anti-Aconitase 1/ACO1 antibody

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(8 Publications)

Rabbit Polyclonal Aconitase 1/ACO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACO1 aa 500-750.

View Alternative Names

IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 antibody (AB236773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 antibody (AB236773)

Paraffin-embedded human kidney tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 antibody (AB236773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 antibody (AB236773)

Paraffin-embedded human small intestine tissue stained for Aconitase 1/ACO1 with ab236773 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 1/ACO1 antibody (AB236773)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 1/ACO1 antibody (AB236773)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 1/ACO1 (Green) using ab236773 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-Aconitase 1/ACO1 antibody (AB236773)
  • WB

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody (AB236773)

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody (ab236773) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACO1 aa 500-750. The exact immunogen used to generate this antibody is proprietary information.

P21399

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 1 also known as ACO1 or ACOONe is an enzyme with a vital role in cellular metabolism. It weighs approximately 98 kDa and is expressed in the cytosol of cells. Mechanically ACO1 functions as a bifunctional protein participating in the citric acid cycle. Its main role is the reversible isomerization of citrate to isocitrate mediated by the dehydration and rehydration processes. ACO1 requires a 4Fe-4S cluster to be active in its enzymatic form influencing cellular iron metabolism by regulating the levels of iron-responsive elements in mRNA.
Biological function summary

ACO1 connects energy production and iron regulation. It binds to iron-responsive elements when the iron is scarce and acts in its aconitase form when iron levels are sufficient. ACO1 is not part of a larger protein complex but plays an important regulatory role in balancing cellular energy output and iron homeostasis. It affects both glycolysis and the electron transport chain indirectly by altering the availability of citric acid cycle intermediates which are important for ATP production.

Pathways

ACO1 integrates into both the citric acid cycle and the iron regulatory pathway. The citric acid cycle also called the Krebs cycle is fundamental for ATP production and ACO1 serves as a pivotal point within this cycle. Moreover through its iron-regulatory function it connects to iron metabolism pathways that are vital for various cellular processes. ACO1 activity also influences other proteins like ferritin by regulating iron storage and transport mechanisms through its dual functionality.

ACO1 plays a role in certain neurodegenerative diseases and anemia. Dysfunction in ACO1's activity can lead to imbalances in iron metabolism contributing to disorders like Friedreich's ataxia where iron accumulation causes mitochondrial damage. Anemia can result from inadequate iron regulation because of faulty ACO1 function affecting proteins such as transferrin which is important for iron transport in the blood. Understanding ACO1's dual regulatory functions provides essential insights into these disease processes and potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed : 1281544, PubMed : 1946430, PubMed : 8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788).. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
See full target information ACO1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406220 PubMed39757767

2025

RTN4IP1 Contributes to ESCC via Regulation of Amino Acid Transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Huifang Wei,Dengyun Zhao,Yafei Zhi,Qiong Wu,Jing Ma,Jialuo Xu,Tingting Liu,Jing Zhang,Penglei Wang,Yamei Hu,Xinyu He,Fangqin Guo,Ming Jiang,Dandan Zhang,Wenna Nie,Ran Yang,Tongjin Zhao,Zigang Dong,Kangdong Liu

Brain sciences 14: PubMed38790419

2024

Annexin 1 Reduces Dermatitis-Induced Itch and Cholestatic Itch through Inhibiting Neuroinflammation and Iron Overload in the Spinal Dorsal Horn of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tang Li,Lingyue Hu,Chao Qin,Yuanjie Li,Zhenhua Song,Yang Jiao,Chunyan Wang,Wei Cui,Linlin Zhang

Environmental toxicology 39:991-1000 PubMed37994395

2023

ASIC1a contributes to the epithelial-mesenchymal transformation of breast cancer by activating the Ca /β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Wang,Chao Yang,Ruihua Yu,Ming Zhuang,Feng Jiang

iScience 26:107377 PubMed37520713

2023

IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Lan,Tao Yang,Qu Yue,Zhao Wang,Xiangyang Zhong,Xin Luo,Boming Zuo,Manqing Zhang,Tianci Zeng,Boyang Liu,Hongbo Guo

Frontiers in cellular neuroscience 16:1007458 PubMed36467611

2022

Attenuated iron stress and oxidative stress may participate in anti-seizure and neuroprotective roles of xenon in pentylenetetrazole-induced epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdi Zhang,Yao Cheng,Yujie Zhai,Yi Yuan,Haoran Hu,Xianfeng Meng,Xuemeng Fan,Hongliu Sun,Shucui Li

Redox report : communications in free radical research 27:119-127 PubMed35735222

2022

Norcantharidin down-regulates iron contents in the liver and spleen of lipopolysaccharide-treated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zheng,Jiao-Jiao Wang,Hui-Min Ma,Meng-Qi Shen,Zhong-Ming Qian,Yu-Xin Bao

Cell death discovery 8:138 PubMed35351859

2022

Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdi Zhang,Yao Cheng,Yujie Zhai,Yaru Cui,Wenshen Zhang,Hongwei Sun,Wenyu Xin,Ling Zhou,Xue Gao,Shucui Li,Hongliu Sun

Oncology letters 22:657 PubMed34386079

2021

Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fengping Yao,Xiaohong Cui,Ying Zhang,Zhuchun Bei,Hongquan Wang,Dongxu Zhao,Hong Wang,Yongfei Yang
View all publications
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