JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183721

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]

Be the first to review this product! Submit a review

|

(7 Publications)

Rabbit Recombinant Monoclonal Aconitase 1/ACO1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

3 Images
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)
  • WB

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

T.T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 98 kDa

false

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)
  • WB

Lab

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

Lanes 1 - 4 : Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/mL

Lane 1:

Wild-type U20S whole cell lysate at 20 µg

Lane 2:

ACO1 knockout U-2 OS whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACO1 knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-aco1-knockout-u-2-os-cell-line-ab261884'>ab261884</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Predicted band size: 98 kDa

false

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)
  • WB

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 98 kDa

false

  • Carrier free

    Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7226(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 1 also known as ACO1 or ACOONe is an enzyme with a vital role in cellular metabolism. It weighs approximately 98 kDa and is expressed in the cytosol of cells. Mechanically ACO1 functions as a bifunctional protein participating in the citric acid cycle. Its main role is the reversible isomerization of citrate to isocitrate mediated by the dehydration and rehydration processes. ACO1 requires a 4Fe-4S cluster to be active in its enzymatic form influencing cellular iron metabolism by regulating the levels of iron-responsive elements in mRNA.
Biological function summary

ACO1 connects energy production and iron regulation. It binds to iron-responsive elements when the iron is scarce and acts in its aconitase form when iron levels are sufficient. ACO1 is not part of a larger protein complex but plays an important regulatory role in balancing cellular energy output and iron homeostasis. It affects both glycolysis and the electron transport chain indirectly by altering the availability of citric acid cycle intermediates which are important for ATP production.

Pathways

ACO1 integrates into both the citric acid cycle and the iron regulatory pathway. The citric acid cycle also called the Krebs cycle is fundamental for ATP production and ACO1 serves as a pivotal point within this cycle. Moreover through its iron-regulatory function it connects to iron metabolism pathways that are vital for various cellular processes. ACO1 activity also influences other proteins like ferritin by regulating iron storage and transport mechanisms through its dual functionality.

ACO1 plays a role in certain neurodegenerative diseases and anemia. Dysfunction in ACO1's activity can lead to imbalances in iron metabolism contributing to disorders like Friedreich's ataxia where iron accumulation causes mitochondrial damage. Anemia can result from inadequate iron regulation because of faulty ACO1 function affecting proteins such as transferrin which is important for iron transport in the blood. Understanding ACO1's dual regulatory functions provides essential insights into these disease processes and potential therapeutic approaches.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed : 1281544, PubMed : 1946430, PubMed : 8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788).. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
See full target information ACO1
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10838 PubMed39738060

2024

Mammalian SLC39A13 promotes ER/Golgi iron transport and iron homeostasis in multiple compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Li,Yanmei Cui,Yule Hu,Mengran Zhao,Kuanyu Li,Xiaoyun Pang,Fei Sun,Bing Zhou

iScience 27:108955 PubMed38322996

2024

Exosomal miR-3174 induced by hypoxia promotes angiogenesis and metastasis of hepatocellular carcinoma by inhibiting HIPK3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Mingyu Wu,Xiangxu Kong,Yun Wang,Chunyang Hu,Deming Zhu,Lianbao Kong,Fei Qiu,Wangjie Jiang

Nature communications 14:3716 PubMed37349299

2023

Mitochondrial aconitase suppresses immunity by modulating oxaloacetate and the mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Eunah Kim,Andrea Annibal,Yujin Lee,Hae-Eun H Park,Seokjin Ham,Dae-Eun Jeong,Younghun Kim,Sangsoon Park,Sujeong Kwon,Yoonji Jung,JiSoo Park,Sieun S Kim,Adam Antebi,Seung-Jae V Lee

Thoracic cancer 14:1477-1491 PubMed37144254

2023

Tumor-derived exosomal miR-148b-3p mediates M2 macrophage polarization via TSC2/mTORC1 to promote breast cancer migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Hao,Zhimei Sheng,Wenhao Wang,Ruijun Feng,Yuanhang Zheng,Qinpei Xiao,Baogang Zhang

Frontiers in pharmacology 14:1127931 PubMed37006994

2023

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis.

Applications

Unspecified application

Species

Unspecified reactive species

Arenbaoligao,Xinrui Guo,Jiahao Xiong,Shuangshuang Zhang,Yuewen Yang,Dapeng Chen,Yu Xie

Scientific reports 12:21483 PubMed36509805

2022

Paraoxonase-2 contributes to promoting lipid metabolism and mitochondrial function via autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gu-Choul Shin,Hyeong Min Lee,Nayeon Kim,Sang-Ku Yoo,Hyung Soon Park,Leo Sungwong Choi,Kwang Pyo Kim,Ah-Ra Lee,Sang-Uk Seo,Kyun-Hwan Kim

Cell reports 35:108983 PubMed33852870

2021

A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giampaolo Morciano,Gaia Pedriali,Massimo Bonora,Rita Pavasini,Elisa Mikus,Simone Calvi,Matteo Bovolenta,Magdalena Lebiedzinska-Arciszewska,Mirko Pinotti,Alberto Albertini,Mariusz R Wieckowski,Carlotta Giorgi,Roberto Ferrari,Lorenzo Galluzzi,Gianluca Campo,Paolo Pinton
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB63260

Anti-Aconitase 1/ACO1 (phospho S138) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 (phospho S138) antibody (AB63260)

  • 0
  • 0
Primary Antibodies

AB236773

Anti-Aconitase 1/ACO1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 1/ACO1 antibody (AB236773)

  • 0
  • 0

Complementary Products

Assay Kits

AB83459

Aconitase Assay Kit

Functional Studies - Aconitase Assay Kit (AB83459)

  • 0
  • 0
Primary Antibodies

AB110321

Anti-Aconitase 2 antibody [6F12BD9]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (AB110321)

  • 5
  • 4
Primary Antibodies

AB214039

Anti-Transferrin Receptor antibody [EPR20584]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] (AB214039)

  • 5
  • 1
Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

  • 4
  • 7
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com