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AB250695

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Aconitase 1/ACO1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

3 Images
Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)
  • WB

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)

This data was developed using ab183721, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (<a href='/en-us/products/primary-antibodies/aconitase-1-aco1-antibody-epr72262-ab183721'>ab183721</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

T.T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 98 kDa

false

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)
  • WB

Lab

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)

This data was developed using ab183721, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (<a href='/en-us/products/primary-antibodies/aconitase-1-aco1-antibody-epr72262-ab183721'>ab183721</a>) at 1 µg/mL

Lane 1:

Wild-type U20S whole cell lysate at 20 µg

Lane 2:

ACO1 knockout U-2 OS whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACO1 knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-aco1-knockout-u-2-os-cell-line-ab261884'>ab261884</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Predicted band size: 98 kDa

false

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)
  • WB

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] - BSA and Azide free (AB250695)

This data was developed using ab183721, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (<a href='/en-us/products/primary-antibodies/aconitase-1-aco1-antibody-epr72262-ab183721'>ab183721</a>) at 1/20000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7226(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250695 is the carrier-free version of ab183721.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 1 also known as ACO1 or ACOONe is an enzyme with a vital role in cellular metabolism. It weighs approximately 98 kDa and is expressed in the cytosol of cells. Mechanically ACO1 functions as a bifunctional protein participating in the citric acid cycle. Its main role is the reversible isomerization of citrate to isocitrate mediated by the dehydration and rehydration processes. ACO1 requires a 4Fe-4S cluster to be active in its enzymatic form influencing cellular iron metabolism by regulating the levels of iron-responsive elements in mRNA.
Biological function summary

ACO1 connects energy production and iron regulation. It binds to iron-responsive elements when the iron is scarce and acts in its aconitase form when iron levels are sufficient. ACO1 is not part of a larger protein complex but plays an important regulatory role in balancing cellular energy output and iron homeostasis. It affects both glycolysis and the electron transport chain indirectly by altering the availability of citric acid cycle intermediates which are important for ATP production.

Pathways

ACO1 integrates into both the citric acid cycle and the iron regulatory pathway. The citric acid cycle also called the Krebs cycle is fundamental for ATP production and ACO1 serves as a pivotal point within this cycle. Moreover through its iron-regulatory function it connects to iron metabolism pathways that are vital for various cellular processes. ACO1 activity also influences other proteins like ferritin by regulating iron storage and transport mechanisms through its dual functionality.

ACO1 plays a role in certain neurodegenerative diseases and anemia. Dysfunction in ACO1's activity can lead to imbalances in iron metabolism contributing to disorders like Friedreich's ataxia where iron accumulation causes mitochondrial damage. Anemia can result from inadequate iron regulation because of faulty ACO1 function affecting proteins such as transferrin which is important for iron transport in the blood. Understanding ACO1's dual regulatory functions provides essential insights into these disease processes and potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed : 1281544, PubMed : 1946430, PubMed : 8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788).. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
See full target information ACO1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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