AB110320

Anti-Aconitase 2 antibody [6D1BE4]

Name: Anti-Aconitase 2 antibody [6D1BE4] (ab110320) | Abcam
Brand: Abcam Limited
SKU: AB110320
Price: 520 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Aconitase 2 antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Aconitase, Citrate hydro-lyase, ACO2

3 Images

Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

Flow Cyt

Unknown

Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

ab110320 at 1μg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note : the target protein locates to the mitochondria.

Lab

Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320.

All lanes:

Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)

Predicted band size: 85 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6D1BE4

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt, IP

applications

Specificity

This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.Purity: >95% by SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.

Biological function summary

Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.

Pathways

Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.

Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

See full target information ACO2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106831 PubMed37250791

2023

Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hu Zhao,Peinan Zhao,Zihao Deng,Ting Yang,Yu-Xing Qi,Li-Ya An,Da-Li Sun,Hai-Yu He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aconitase 2 antibody [6D1BE4]

Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (AB110320)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise