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Mouse Monoclonal Aconitase 2 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 31 publications.


Images

Flow Cytometry - Anti-Aconitase 2 antibody [6F12BD9] (AB110321), expandable thumbnail
  • Western blot - Anti-Aconitase 2 antibody [6F12BD9] (AB110321), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (AB110321), expandable thumbnail
  • Western blot - Anti-Aconitase 2 antibody [6F12BD9] (AB110321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [6F12BD9] (AB110321), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

for 2 hours.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Alternative names

Aconitase, Citrate hydro-lyase, ACO2

Recommended products

Mouse Monoclonal Aconitase 2 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 31 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
6F12BD9
Light chain type
kappa
Concentration
Loading...
Purification notes

ab110321 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.Purity: >95% by SDS-PAGE.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.

Biological function summary

Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.

Pathways

Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.

Associated diseases and disorders

Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321), expandable thumbnail

    Flow Cytometry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    ab110321 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

  • Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321), expandable thumbnail

    Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)

    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab110321 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab110321 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab110321 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    Predicted band size: 85 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (ab110321), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    ab110321 at 1μg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).

  • Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321), expandable thumbnail

    Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    All lanes: Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321) at 1 µg/mL

    Lane 1: HepG2 (human) at 20 µg

    Lane 2: HeLa (human) at 20 µg

    Lane 3: H9C2 (rat cardiomyocyte) at 20 µg

    Lane 4: MEF (mouse fibroblast) at 20 µg

    Predicted band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    IHC image of Aconitase 2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110321, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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Product protocols

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