Rabbit Polyclonal Aconitase 2 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACO2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACO2. The exact immunogen used to generate this antibody is proprietary information. Database link Q99798
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Expected | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/20000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/20000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Alternative names
Aconitase, Citrate hydro-lyase, ACO2
Recommended products
Rabbit Polyclonal Aconitase 2 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACO2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACO2. The exact immunogen used to generate this antibody is proprietary information. Database link Q99798
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.
- Biological function summary
Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.
- Pathways
Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.
- Associated diseases and disorders
Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
Western blot - Anti-Aconitase 2 antibody (ab228923)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa
Western blot - Anti-Aconitase 2 antibody (ab228923)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution
All lanes: Rat brain extract at 50 µg
Predicted band size: 85 kDa
Immunoprecipitation - Anti-Aconitase 2 antibody (ab228923)
Aconitase 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab228923. Western blot was performed from the immunoprecipitate using ab228923. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HeLa whole cell extract.
Lane 2: ab228923 IP in HeLa whole cell extract.
All lanes: Immunoprecipitation - Anti-Aconitase 2 antibody (ab228923)
Predicted band size: 85 kDa
Western blot - Anti-Aconitase 2 antibody (ab228923)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution
All lanes: Mouse brain lysate at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa
Western blot - Anti-Aconitase 2 antibody (ab228923)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution
All lanes: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)
Paraffin-embedded mouse liver tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody (ab228923)
2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 2 (green) using ab228923 at 1/1000 dilution in ICC/IF.
Red: A mitochondria tracker.
Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)
Paraffin-embedded mouse kidney tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)
Paraffin-embedded mouse urinary bladder tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab228923)
Paraffin-embedded mouse intestine tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com