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Rabbit Recombinant Monoclonal Aconitase 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 21 publications.


Images

Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (AB129069), expandable thumbnail
  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (AB129069), expandable thumbnail
  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (AB129069), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (AB129069), expandable thumbnail
  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (AB129069), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Predicted
Not recommended
Predicted
Rat
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Alternative names

Aconitase, Citrate hydro-lyase, ACO2

Recommended products

Rabbit Recombinant Monoclonal Aconitase 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 21 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8282(B)
Purification technique
Affinity purification Protein A
Dissociation constant
2.9 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.

Biological function summary

Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.

Pathways

Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.

Associated diseases and disorders

Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069), expandable thumbnail

    Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    Predicted band size: 85 kDa

  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069), expandable thumbnail

    Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    All lanes: Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: Human fetal heart tissue lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 85 kDa

  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069), expandable thumbnail
    This image is courtesy of a customer review submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    Blocking: 5% Milk at 24°C for 1 Hour

    All lanes: Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/1000 dilution

    Lane 1: 1 Month old Mouse Liver

    Lanes 2 - 3: 3 Month old Mouse Liver

    Lane 4: 6 Month old Mouse Liver

    Secondary

    All lanes: Dako Pig polylconal to anti-rabbit (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069), expandable thumbnail

    OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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