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AB100915

Anti-ACOT1 antibody

3

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal ACOT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT1 aa 150 to C-terminus.

View Alternative Names

CTE1, ACOT1, Acyl-coenzyme A thioesterase 1, Acyl-CoA thioesterase 1, CTE-I, CTE-Ib, Inducible cytosolic acyl-coenzyme A thioester hydrolase, Long chain acyl-CoA thioester hydrolase, Palmitoyl-coenzyme A thioesterase, Long chain acyl-CoA hydrolase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOT1 antibody (AB100915)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOT1 antibody (AB100915)

ab100915, at 1/100 dilution, staining cytoplasmic ACOT1 in formalin-fixed, paraffin-embedded Human fetal thymus tissue, by Immunohistochemistry.

Western blot - Anti-ACOT1 antibody (AB100915)
  • WB

Unknown

Western blot - Anti-ACOT1 antibody (AB100915)

All lanes:

Western blot - Anti-ACOT1 antibody (ab100915) at 1/1000 dilution

Lane 1:

Human Fetal Skin Lysate

Lane 2:

Human Fetal Liver Lysate

Lane 3:

Human Fetal Heart Lysate

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACOT1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86TX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACOT1 also known as Acyl-CoA thioesterase 1 is an enzyme involved in the hydrolysis of acyl-CoA to free fatty acid and CoA. It has a molecular mass of approximately 46 kDa. ACOT1 expresses mainly in liver kidney and heart tissues where it regulates levels of acyl-CoA an important molecule in various metabolic processes. Its activity facilitates the balance between thioesterase and transferase reactions impacting fatty acid metabolism.
Biological function summary

ACOT1 plays a role in lipid metabolism by managing the acyl-CoA pool. This enzyme helps in modulating the intracellular concentration of acyl-CoA and free fatty acids which are important for energy production and storage. It does not integrate into a larger protein complex instead functioning independently within cells. The enzyme's ability to control lipid and energy metabolism ties it to important biological processes.

Pathways

ACOT1 participates in fatty acid degradation and peroxisome proliferator-activated receptor (PPAR) signaling pathways. In these pathways ACOT1 works alongside proteins such as enzymes involved in beta-oxidation where it aids in the efficient breakdown and utilization of fatty acids. PPAR signaling influences energy balance and metabolism making ACOT1 integral to these pathways and linked with proteins like PPAR-alpha that regulate lipid metabolism.

ACOT1 has associations with metabolic syndrome and nonalcoholic fatty liver disease (NAFLD). Alterations in ACOT1 expression or activity could disrupt lipid metabolism leading to these conditions. In NAFLD changes in ACOT1 can result in excess free fatty acids affecting liver function. The enzyme connects with proteins like acetyl-CoA carboxylase which plays a part in lipid metabolism disorders demonstrating its role in these health problems.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).
See full target information ACOT1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102700 PubMed34235408

2021

Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Shadrach,Wesley M Stansberry,Allison M Milen,Rachel E Ives,Elizabeth A Fogarty,Anthony Antonellis,Brian A Pierchala

Cell death & disease 11:756 PubMed32934217

2020

Acyl-CoA thioesterase 1 prevents cardiomyocytes from Doxorubicin-induced ferroptosis via shaping the lipid composition.

Applications

Unspecified application

Species

Unspecified reactive species

Yunchang Liu,Liping Zeng,Yong Yang,Chen Chen,Daowen Wang,Hong Wang

Cell 169:1051-1065.e18 PubMed28575669

2017

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria Barna

Nutrition research (New York, N.Y.) 34:694-706 PubMed25172377

2014

Dietary fatty acids alter left ventricular myocardial gene expression in Wistar rats.

Applications

WB

Species

Rat

Kimberly M Jeckel,Gerrit J Bouma,Ann M Hess,Erin B Petrilli,Melinda A Frye
View all publications
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