AB85151

Anti-ACOT7 antibody

Name: Anti-ACOT7 antibody (ab85151) | Abcam
Brand: Abcam Limited
SKU: AB85151

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(3 Publications)

Rabbit Polyclonal ACOT7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ACOT7.

View Alternative Names

BACH, ACOT7, Cytosolic acyl coenzyme A thioester hydrolase, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, CTE-IIa, Long chain acyl-CoA thioester hydrolase, hBACH, CTE-II

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOT7 antibody (AB85151)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOT7 antibody (AB85151)

ab85151, at a 1/100 dilution, staining ACOT7 in formalin fixed, paraffin embedded human brain tissue by Immunohistochemistry.

Unknown

Western blot - Anti-ACOT7 antibody (AB85151)

All lanes:

Western blot - Anti-ACOT7 antibody (ab85151) at 1/500 dilution

All lanes:

human fetal brain lysate

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACOT7. The exact immunogen used to generate this antibody is proprietary information.

O00154

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACOT7 also known as cytosolic acyl-CoA thioesterase 7 plays an important role in lipid metabolism by hydrolyzing acyl-CoA thioesters into free fatty acids and CoA. This enzyme possesses a molecular mass of approximately 44 kilodaltons. ACOT7 primarily expresses in the brain with significant levels in neurons. This distribution suggests involvement in processes related to brain lipid metabolism and energy homeostasis.

Biological function summary

ACOT7 influences the regulation of lipid pools and fatty acid signaling in neural cells. It participates in the modulation of free fatty acid concentrations affecting energy metabolism and neuronal function. While ACOT7 does not typically associate with larger protein complexes its enzymatic action impacts the availability and signaling of lipid molecules within the cell potentially influencing cellular responses and energy dynamics.

Pathways

ACOT7 plays a role in fatty acid metabolism and energy homeostasis pathways. It connects with lipid metabolism processes involving enzymes like fatty acid synthase and acyl-CoA synthetase enzymes. By modulating the levels of acyl-CoA and free fatty acids ACOT7 affects broader metabolic pathways that control cellular energy balance and lipid synthesis in brain tissues.

ACOT7 links to neurological conditions and energy metabolism disorders. Its alteration associates with neurodegenerative diseases such as Alzheimer's disease due to its role in maintaining neuronal lipid balance. Furthermore conditions like metabolic syndrome may relate to changes in ACOT7 function given its impact on lipid metabolism. Proteins like amyloid-beta precursor protein in Alzheimer's disease have connections to ACOT7 through these metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 10578051). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed : 10578051). May play an important physiological function in brain (PubMed : 10578051).

See full target information ACOT7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 8:e2793 PubMed28518146

2017

Acyl-CoA thioesterase 7 is involved in cell cycle progression via regulation of PKCζ-p53-p21 signaling pathway.

Applications

Species

Human

Seung Hee Jung,Hyung Chul Lee,Hyun Jung Hwang,Hyun A Park,Young-Ah Moon,Bong Cho Kim,Hyeong Min Lee,Kwang Pyo Kim,Yong-Nyun Kim,Byung Lan Lee,Jae Cheol Lee,Young-Gyu Ko,Heon Joo Park,Jae-Seon Lee

Journal of lipid research 58:1174-1185 PubMed28416579

2017

Inflammatory stimuli induce acyl-CoA thioesterase 7 and remodeling of phospholipids containing unsaturated long (≥C20)-acyl chains in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Z Wall,Shelley Barnhart,Farah Kramer,Jenny E Kanter,Anuradha Vivekanandan-Giri,Subramaniam Pennathur,Chiara Bolego,Jessica M Ellis,Miguel A Gijón,Michael J Wolfgang,Karin E Bornfeldt

PloS one 10:e0138366 PubMed26379276

2015

Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation.

Applications

IHC, WB, IF

Species

Unspecified reactive species, Unspecified reactive species, Unspecified reactive species

Vanessa Montoya,Hanli Fan,Paul J Bryar,Joanna L Weinstein,Marilyn B Mets,Gang Feng,Joshua Martin,Alissa Martin,Hongmei Jiang,Nikia A Laurie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACOT7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACOT7 antibody (AB85151)

Western blot - Anti-ACOT7 antibody (AB85151)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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