AB249270

Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ACOT7 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

BACH, ACOT7, Cytosolic acyl coenzyme A thioester hydrolase, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, CTE-IIa, Long chain acyl-CoA thioester hydrolase, hBACH, CTE-II

2 Images

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Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

This data was developed using ab156576, the same antibody clone in a different buffer formulation.WB analysis of immunoprecipites
ab156576, at a 1/1000 dilution, staining ACOT7 in Jurkat cell lysate immunoprecipitated using ab156576 at 1/100 dilution.

All lanes:

Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] (<a href='/en-us/products/primary-antibodies/acot7-antibody-epr11317b-ab156576'>ab156576</a>)

Predicted band size: 42 kDa

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Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

This data was developed using ab156576, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ACOT7 antibody [EPR11317(B)] (<a href='/en-us/products/primary-antibodies/acot7-antibody-epr11317b-ab156576'>ab156576</a>) at 1/1000 dilution

Lane 1:

fetal brain cell lysate at 10 µg/mL

Lane 2:

A375 cell lysate at 10 µg/mL

Lane 3:

293T cell lysate at 10 µg/mL

Lane 4:

Jurkat cell lysate at 10 µg/mL

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

Unconjugated

Anti-ACOT7 antibody [EPR11317(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11317(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249270 is the carrier-free version of ab156576.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACOT7 also known as cytosolic acyl-CoA thioesterase 7 plays an important role in lipid metabolism by hydrolyzing acyl-CoA thioesters into free fatty acids and CoA. This enzyme possesses a molecular mass of approximately 44 kilodaltons. ACOT7 primarily expresses in the brain with significant levels in neurons. This distribution suggests involvement in processes related to brain lipid metabolism and energy homeostasis.

Biological function summary

ACOT7 influences the regulation of lipid pools and fatty acid signaling in neural cells. It participates in the modulation of free fatty acid concentrations affecting energy metabolism and neuronal function. While ACOT7 does not typically associate with larger protein complexes its enzymatic action impacts the availability and signaling of lipid molecules within the cell potentially influencing cellular responses and energy dynamics.

Pathways

ACOT7 plays a role in fatty acid metabolism and energy homeostasis pathways. It connects with lipid metabolism processes involving enzymes like fatty acid synthase and acyl-CoA synthetase enzymes. By modulating the levels of acyl-CoA and free fatty acids ACOT7 affects broader metabolic pathways that control cellular energy balance and lipid synthesis in brain tissues.

ACOT7 links to neurological conditions and energy metabolism disorders. Its alteration associates with neurodegenerative diseases such as Alzheimer's disease due to its role in maintaining neuronal lipid balance. Furthermore conditions like metabolic syndrome may relate to changes in ACOT7 function given its impact on lipid metabolism. Proteins like amyloid-beta precursor protein in Alzheimer's disease have connections to ACOT7 through these metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 10578051). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed : 10578051). May play an important physiological function in brain (PubMed : 10578051).

See full target information ACOT7

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free

Immunoprecipitation - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

Western blot - Anti-ACOT7 antibody [EPR11317(B)] - BSA and Azide free (AB249270)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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