Rabbit Recombinant Monoclonal ACOX1/AOX antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Predicted | Expected | Predicted | Predicted |
Mouse | Tested | Expected | Tested | Tested |
Rat | Predicted | Expected | Predicted | Predicted |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids (PubMed:15060085, PubMed:17458872, PubMed:17603022, PubMed:32169171, PubMed:33234382, PubMed:7876265). Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)) (PubMed:17458872, PubMed:17603022, PubMed:7876265).Isoform 1Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain length of 10 carbons (decanoyl-CoA) in vitro.Isoform 2Is active against a much broader range of substrates and shows activity towards long-chain fatty acyl-CoAs.
ACOX, ACOX1, ACOX, Peroxisomal acyl-coenzyme A oxidase 1, AOX, Palmitoyl-CoA oxidase, Peroxisomal fatty acyl-CoA oxidase, Straight-chain acyl-CoA oxidase, SCOX
Rabbit Recombinant Monoclonal ACOX1/AOX antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR19038
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab238939 is the carrier-free version of Anti-ACOX1/AOX antibody [EPR19038] ab184032.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
ACOX1 also known as AOX or AOX animal plays an important role in peroxisomal beta-oxidation of fatty acids. This enzyme catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. ACOX1 with a mass of approximately 73 kDa is primarily expressed in liver and kidney tissues where it contributes to lipid metabolism. The functionality of this protein is essential for maintaining the balance of fatty acids within the peroxisome which is an important organelle in cellular lipid homeostasis.
ACOX1 functions as part of the fatty acid oxidation process by ensuring the efficient breakdown of very long-chain fatty acids. This enzyme operates in conjunction with other proteins involved in the beta-oxidation enzyme complex. The activity of ACOX1 is significant for energy production metabolizing long-chain fatty acids which accumulate under normal physiological conditions. Proper function of this enzyme ensures that excessive accumulation of these fatty acids does not occur which can otherwise lead to cellular stress or dysfunction.
ACOX1 fits into lipid metabolism pathways specifically the peroxisomal beta-oxidation pathway. This pathway is critical for the breakdown of long and very long-chain fatty acids. ACOX1 functions together with other peroxisomal enzymes such as peroxisomal acyl-coenzyme A oxidase 2 (ACOX2) to complete this metabolic process. Both these enzymes work in tandem to manage fatty acid levels within the peroxisomal pathway ensuring smooth cellular energy homeostasis and overall metabolic function.
Mutations or deficiencies in ACOX1 relate closely to conditions like adrenoleukodystrophy and Zellweger syndrome. These disorders arise from impaired peroxisomal function leading to the accumulation of very long-chain fatty acids that are typically processed by ACOX1. Such accumulations can exacerbate neurological and hepatic disorders. Additionally a dysfunctional interaction between ACOX1 and peroxisomal protein peroxin 1 (PEX1) can result in compromised peroxisome function further contributing to the pathology of these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ACOX1/AOX with Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACOX1/AOX antibody [EPR19038] ab184032).
ACOX1/AOX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10μg (Input).
Lane 2: Anti-ACOX1/AOX antibody [EPR19038] ab184032 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR19038] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ACOX1/AOX antibody [EPR19038] ab184032 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACOX1/AOX antibody [EPR19038] ab184032).
All lanes: Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] (Anti-ACOX1/AOX antibody [EPR19038] ab184032)
Predicted band size: 74 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ACOX1/AOX with Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACOX1/AOX antibody [EPR19038] ab184032).
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast) cell linelabeling ACOX1/AOX with Anti-ACOX1/AOX antibody [EPR19038] ab184032 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR19038] - Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ACOX1/AOX antibody [EPR19038] ab184032).
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