Skip to main content

Rabbit Recombinant Monoclonal ACOX1/AOX antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939), expandable thumbnail
  • Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Predicted
Expected
Predicted
Predicted
Mouse
Tested
Expected
Tested
Tested
Rat
Predicted
Expected
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Target data

Function

Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids (PubMed:15060085, PubMed:17458872, PubMed:17603022, PubMed:32169171, PubMed:33234382, PubMed:7876265). Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O(2)), thereby producing hydrogen peroxide (H(2)O(2)) (PubMed:17458872, PubMed:17603022, PubMed:7876265).Isoform 1Shows highest activity against medium-chain fatty acyl-CoAs. Shows optimum activity with a chain length of 10 carbons (decanoyl-CoA) in vitro.Isoform 2Is active against a much broader range of substrates and shows activity towards long-chain fatty acyl-CoAs.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ACOX1/AOX antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR19038

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab238939 is the carrier-free version of Anti-ACOX1/AOX antibody [EPR19038] ab184032.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ACOX1 also known as AOX or AOX animal plays an important role in peroxisomal beta-oxidation of fatty acids. This enzyme catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. ACOX1 with a mass of approximately 73 kDa is primarily expressed in liver and kidney tissues where it contributes to lipid metabolism. The functionality of this protein is essential for maintaining the balance of fatty acids within the peroxisome which is an important organelle in cellular lipid homeostasis.

Biological function summary

ACOX1 functions as part of the fatty acid oxidation process by ensuring the efficient breakdown of very long-chain fatty acids. This enzyme operates in conjunction with other proteins involved in the beta-oxidation enzyme complex. The activity of ACOX1 is significant for energy production metabolizing long-chain fatty acids which accumulate under normal physiological conditions. Proper function of this enzyme ensures that excessive accumulation of these fatty acids does not occur which can otherwise lead to cellular stress or dysfunction.

Pathways

ACOX1 fits into lipid metabolism pathways specifically the peroxisomal beta-oxidation pathway. This pathway is critical for the breakdown of long and very long-chain fatty acids. ACOX1 functions together with other peroxisomal enzymes such as peroxisomal acyl-coenzyme A oxidase 2 (ACOX2) to complete this metabolic process. Both these enzymes work in tandem to manage fatty acid levels within the peroxisomal pathway ensuring smooth cellular energy homeostasis and overall metabolic function.

Associated diseases and disorders

Mutations or deficiencies in ACOX1 relate closely to conditions like adrenoleukodystrophy and Zellweger syndrome. These disorders arise from impaired peroxisomal function leading to the accumulation of very long-chain fatty acids that are typically processed by ACOX1. Such accumulations can exacerbate neurological and hepatic disorders. Additionally a dysfunctional interaction between ACOX1 and peroxisomal protein peroxin 1 (PEX1) can result in compromised peroxisome function further contributing to the pathology of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com