Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast) cell linelabeling ACOX1/AOX with ab184032 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR19038] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

• IP

Supplier Data

Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] - BSA and Azide free (AB238939)

ACOX1/AOX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab184032 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184032 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : NIH/3T3 whole cell lysate 10μg (Input).
Lane 2 : ab184032 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit IgG, monoclonal [EPR19038] - Isotype Control (ab172730) instead of ab184032 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184032).

All lanes:

Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] (<a href='/en-us/products/primary-antibodies/acox1-aox-antibody-epr19038-ab184032'>ab184032</a>)

Predicted band size: 74 kDa

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