Anti-Acrolein antibody [10A10]

• IHC

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Immunohistochemistry - Anti-Acrolein antibody [10A10] (AB240918)

Immunohistochemistry analysis labeling human adrenal carcinoma tissue with ab240918 at a 1/100 dilution. Tissue samples were incubated with the primary antibody ab240918 at a 1/100 dilution overnight at 4^oC. A Goat anti-Mouse IgG (H&L) : FITC secondary was used (45 min at 37^oC). Tissue samples were counterstained with DAPI (blue) for 3 minutes at room temperature. Magification 5X.

• Flow Cyt

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Flow Cytometry - Anti-Acrolein antibody [10A10] (AB240918)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Acrolein using ab240918 at 1/50 dilution for 30 minutes on ice (solid line) compared to a non-specific IgG control (dashed line). Secondary antibody was a PE-conjugated goat anti-mouse IgG at 1/100 dilution for 20 minutes at room temperature. Cells were subject to oxidative stress by treating with 250 μM H₂O₂ for 24 hours.

• WB

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Western blot - Anti-Acrolein antibody [10A10] (AB240918)

Block : 5% Skim Milk in TBST.

Primary incubation for 2 hours at RT.

All lanes:

Western blot - Anti-Acrolein antibody [10A10] (ab240918) at 1/1000 dilution

Lane 1:

Acrolein-BSA at 0.5 µg

Lane 2:

Acrolein-BSA at 2 µg

Lane 3:

BSA at 0.5 µg

Lane 4:

BSA at 2 µg

Secondary

All lanes:

HRP-linked goat anti-mouse IgG at 1/2000 dilution

true

Exposure time: 5min

• WB

Supplier Data

Western blot - Anti-Acrolein antibody [10A10] (AB240918)

Block : 5% Skim Milk in TBST.

Primary incubation for 2 hours at RT.

All lanes:

Western blot - Anti-Acrolein antibody [10A10] (ab240918) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

Hydrogen peroxide-treated HeLa cell lysate at 12 µg

Secondary

All lanes:

HRP-linked goat anti-mouse IgG at 1/2000 dilution

true

Exposure time: 5min