Anti-Acrolein antibody [2H2]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Acrolein antibody [2H2] (AB240906)

Flow cytometric analysis of 90% Methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells using ab240906 at 1/50 dilution for 30 minutes on ice (solid line) compared to a control IgG (dotted line). Goat Anti-Mouse PE at 1/100 dilution for 20 min at RT was used as the secondary antibody.

Cells were subject to oxidative stress by treating with 250 μM H₂O₂ for 24 hours.

• WB

Supplier Data

Western blot - Anti-Acrolein antibody [2H2] (AB240906)

Block : 5% Skim Milk in 1X TBST.

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

All lanes:

Western blot - Anti-Acrolein antibody [2H2] (ab240906) at 1/1000 dilution

Lane 1:

ab245924 at 1 µg

Lane 2:

ab245924 at 0.5 µg

Lane 3:

ab245924 at 0.1 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

Predicted band size: 69 kDa

true

Exposure time: 5min

• WB

Supplier Data

Western blot - Anti-Acrolein antibody [2H2] (AB240906)

All lanes:

Western blot - Anti-Acrolein antibody [2H2] (ab240906) at 1/1000 dilution

Lane 1:

Acrolein-BSA at 0.5 µg

Lane 2:

Acrolein-BSA at 2 µg

Lane 3:

BSA at 0.5 µg

Lane 4:

BSA at 2 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT

true

Exposure time: 5min