Rabbit Recombinant Monoclonal ACSL1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 12 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95235.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92440.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)")
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img61392.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)")
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img435278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)")
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img442638.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)")
Publications
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- PeerJ 11:e166592023CXCL10-based gene cluster model serves as a potential diagnostic biomarker for premature ovarian failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Qin,Canliang Wen,Huijiao WuPubMed 38107572
- Histology and histopathology 39:239-2502023TRIM3 inhibits colorectal cancer cell migration and lipid droplet formation by promoting FABP4 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Zuo,Qimei Xu,Zhen Li,Dixian Luo,Hanwu Peng,Zhi DuanPubMed 37212515
- Scientific reports 12:179902022ACSL1 promotes imatinib-induced chronic myeloid leukemia cell senescence by regulating SIRT1/p53/p21 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Liu,Xiaoying Zhu,Ling Tang,Na Shen,Fanjun Cheng,Ping Zou,Yong You,Guolin Yuan,Qing Li,Xiaojian ZhuPubMed 36289272
- Bioactive materials 16:82-942022Adoptive transfer of metabolically reprogrammed macrophages for atherosclerosis treatment in diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Wang,Yan Dong,Li Yao,Fan Lu,Chenxi Wen,Zhuo Wan,Li Fan,Zhelong Li,Te Bu,Mengying Wei,Xuekang Yang,Yi ZhangPubMed 35386323
- Journal of cellular and molecular medicine 25:8863-88762021Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhixiao Luo,Yaqi Wang,Mengzhen Xue,Fangqi Xia,Leiqi Zhu,Yuanyang Li,Dengke Jia,Silong Chen,Guangfu Xu,Yan LeiPubMed 34402182
- Frontiers in endocrinology 12:6391652021Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Sepsis-Induced Acute Kidney Injury by Promoting Mitophagy of Renal Tubular Epithelial Cells the SIRT1/Parkin Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Guo,Rong Wang,Donghai LiuPubMed 34248837
- PloS one 16:e02462792021Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJilong Han et. al.PubMed 33529214
- Cell death & disease 11:2332020MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1α/CEBPB axis to promote stemness and chemo-resistance of gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHonglei Wu et. al.PubMed 32300102
- International journal of medical sciences 17:720-7272020ACSL1 affects Triglyceride Levels through the PPARγ Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Li et. al.PubMed 32218693
- Biology of reproduction 101:126-1372019Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway†.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Che,Mengmeng Xu,Kaiguo Gao,Cui Zhu,Li Wang,Xuefen Yang,Xiaolu Wen,Hao Xiao,Zongyong Jiang,De WuPubMed 30985894
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
Additional Targets
ACSL6
Alternative names
FACL1, FACL2, LACS, LACS1, LACS2, ACSL1, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, Phytanate--CoA ligase, ACS1, LACS 1, LACS 2
Recommended products
Rabbit Recombinant Monoclonal ACSL1 antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13499
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACSL1 and ACSL6 are members of the acyl-CoA synthetase long-chain family. ACSL1 is also known as Acyl-CoA synthetase 1 with a mass of approximately 78 kDa. ACSL6 goes by the name Acyl-CoA synthetase 6. These enzymes convert free long-chain fatty acids into their active form acyl-CoA. ACSL1 is expressed in tissues like liver heart and adipose tissue while ACSL6 shows expression in brain and leukocytes. The activity of these enzymes is essential for lipid metabolism and energy production processes.
- Biological function summary
The acyl-CoA synthetase functions as a gatekeeper in cellular lipid metabolism. Both ACSL1 and ACSL6 belong to a multi-enzyme complex involved in lipid modification processes. ACSL1 supports triglyceride synthesis and beta-oxidation of fatty acids. This role impacts energy homeostasis and lipid signaling. ACSL6 on the other hand participates in the formation of the myelin sheath in neurons and also influences neuronal differentiation through its action on fatty acid substrates.
- Pathways
The enzymatic actions of ACSL1 and ACSL6 integrate into critical metabolic pathways. ACSL1 plays a role in the beta-oxidation pathway working in conjunction with enzymes like CPT1 to facilitate the transportation of acyl-CoA into mitochondria. ACSL6 is involved in the synthesis of myelin basic protein through the regulation of fatty acid availability. These enzymes also have connections to the peroxisome proliferator-activated receptor (PPAR) signaling pathway which controls the expression of genes related to lipid metabolism.
- Associated diseases and disorders
ACSL1 and ACSL6 have roles in metabolic and neurological diseases. ACSL1 is linked to metabolic diseases such as insulin resistance and type 2 diabetes as impaired lipid metabolism can affect insulin signaling pathways. ACSL6 associates with neurological disorders including schizophrenia due to its role in membrane lipid composition in neurons. The relationship of ACSL6 with proteins like myelin basic protein in the central nervous system underlines its importance in maintaining normal cognitive functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95235.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)
Immunohistochemical analysis of Human liver tissue labeling ACSL1 + ACSL6 using ab177958 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92440.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)
Immunohistochemical analysis of Human kidney tissue labeling ACSL1 + ACSL6 using ab177958 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img61392.jpg "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)")
Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)
All lanes: Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
Predicted band size: 78 kDa
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img435278.jpg "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)")
Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/1000000 dilution.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 was used at 1/1000 dilution.
All lanes: Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector whole cell lysate at 10 µg
Lane 2: 293T transfected with a human ACSL1 expression vector containing a DDDDK tag whole cell lysate at 10 µg
Lane 3: 293T transfected with a human ACSL5 expression vector containing a DDDDK tag whole cell lysate at 10 µg
Lane 4: 293T transfected with a human ACSL6 expression vector containing a DDDDK tag whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution
Observed band size: 75 kDa
Exposure time: 3s
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-ab177958--western-blot-img442638.jpg "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (ab177958)
ACSL1 + ACSL6 western blot using anti-ACSL1 + ACSL6 antibody [EPR13499] ab177958. Publication image and figure legend from Li, T., Li, X., et al., 2020, Int J Med Sci, PubMed 32218693.
ab177958 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab177958 please see the product overview.
Comparison of expression level of ACSL1 gene at the protein level. Sample 1 is the overexpression control group, sample 2 is the overexpression group, sample 3 is the knockdown group, and sample 4 is the knockdown control group.
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