Rabbit Recombinant Monoclonal ACSL1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.
Images
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] - BSA and Azide free (AB250028), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-bsa-and-azide-free-ab250028--western-blot-img435278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] - BSA and Azide free (AB250028)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
Additional Targets
ACSL6
Alternative names
FACL1, FACL2, LACS, LACS1, LACS2, ACSL1, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, Phytanate--CoA ligase, ACS1, LACS 1, LACS 2
Recommended products
Rabbit Recombinant Monoclonal ACSL1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13499
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250028 is the carrier-free version of Anti-ACSL1 + ACSL6 antibody [EPR13499] ab177958.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACSL1 and ACSL6 are members of the acyl-CoA synthetase long-chain family. ACSL1 is also known as Acyl-CoA synthetase 1 with a mass of approximately 78 kDa. ACSL6 goes by the name Acyl-CoA synthetase 6. These enzymes convert free long-chain fatty acids into their active form acyl-CoA. ACSL1 is expressed in tissues like liver heart and adipose tissue while ACSL6 shows expression in brain and leukocytes. The activity of these enzymes is essential for lipid metabolism and energy production processes.
- Biological function summary
The acyl-CoA synthetase functions as a gatekeeper in cellular lipid metabolism. Both ACSL1 and ACSL6 belong to a multi-enzyme complex involved in lipid modification processes. ACSL1 supports triglyceride synthesis and beta-oxidation of fatty acids. This role impacts energy homeostasis and lipid signaling. ACSL6 on the other hand participates in the formation of the myelin sheath in neurons and also influences neuronal differentiation through its action on fatty acid substrates.
- Pathways
The enzymatic actions of ACSL1 and ACSL6 integrate into critical metabolic pathways. ACSL1 plays a role in the beta-oxidation pathway working in conjunction with enzymes like CPT1 to facilitate the transportation of acyl-CoA into mitochondria. ACSL6 is involved in the synthesis of myelin basic protein through the regulation of fatty acid availability. These enzymes also have connections to the peroxisome proliferator-activated receptor (PPAR) signaling pathway which controls the expression of genes related to lipid metabolism.
- Associated diseases and disorders
ACSL1 and ACSL6 have roles in metabolic and neurological diseases. ACSL1 is linked to metabolic diseases such as insulin resistance and type 2 diabetes as impaired lipid metabolism can affect insulin signaling pathways. ACSL6 associates with neurological disorders including schizophrenia due to its role in membrane lipid composition in neurons. The relationship of ACSL6 with proteins like myelin basic protein in the central nervous system underlines its importance in maintaining normal cognitive functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] - BSA and Azide free (ab250028), expandable thumbnail](https://content.abcam.com/products/images/acsl1-acsl6-antibody-epr13499-bsa-and-azide-free-ab250028--western-blot-img435278.jpg "Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] - BSA and Azide free (ab250028)")
Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] - BSA and Azide free (ab250028)
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/1000000 dilution.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 was used at 1/1000 dilution.
All lanes: Western blot - Anti-ACSL1 + ACSL6 antibody [EPR13499] (Anti-ACSL1 + ACSL6 antibody [EPR13499] ab177958) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector whole cell lysate at 10 µg
Lane 2: 293T transfected with a human ACSL1 expression vector containing a DDDDK tag whole cell lysate at 10 µg
Lane 3: 293T transfected with a human ACSL5 expression vector containing a DDDDK tag whole cell lysate at 10 µg
Lane 4: 293T transfected with a human ACSL6 expression vector containing a DDDDK tag whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution
Observed band size: 75 kDa
Exposure time: 3s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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