JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB189939

Anti-ACSL1 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal ACSL1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ACSL1.

View Alternative Names

FACL1, FACL2, LACS, LACS1, LACS2, ACSL1, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, Phytanate--CoA ligase, ACS1, LACS 1, LACS 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ACSL1 antibody (AB189939)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACSL1 antibody (AB189939)

Immunofluorescent analysis of Human lung tissue, staining ACSL1 with ab189939 at 20 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody (AB189939)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody (AB189939)

Immunohistochemistry of formalin-fixed, paraffin-embedded Human kidney tissue labeling ACSL1 with ab189939 at 5 μg/ml.

Western blot - Anti-ACSL1 antibody (AB189939)
  • WB

Supplier Data

Western blot - Anti-ACSL1 antibody (AB189939)

Lane 1:

Western blot - Anti-ACSL1 antibody (ab189939) at 1 µg

Lane 2:

Western blot - Anti-ACSL1 antibody (ab189939) at 2 µg

All lanes:

Human lung tissue

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ACSL1. The exact immunogen used to generate this antibody is proprietary information.

P33121

Specificity

At least three isoforms of ACSL1 are known to exist; ab189939 will detect all three isoforms.

style
left-column

Alternative Products

Primary Antibodies

AB177958

Anti-ACSL1 + ACSL6 antibody [EPR13499]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 + ACSL6 antibody [EPR13499] (AB177958)

  • 4
  • 1
Primary Antibodies

AB178419

Anti-ACSL1 antibody [EPR13498]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (AB178419)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB110284

Anti-HADH antibody [1A12BC8]

Immunocytochemistry/ Immunofluorescence - Anti-HADH antibody [1A12BC8] (AB110284)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme ACSL1 known as Acyl-CoA synthetase long-chain family member 1 plays an important role in lipid metabolism by converting free long-chain fatty acids into fatty acyl-CoA esters. This reaction is essential for the β-oxidation and synthesis of complex lipids. ACSL1 has an approximate mass of 78 kDa and is expressed in tissues with high metabolic activity including the liver heart and adipose tissue. The enzyme functions by regulating the cellular levels of fatty acids and their derivatives significantly impacting energy homeostasis.
Biological function summary

ACSL1 affects energy production and lipid storage by activating fatty acids making them substrates for metabolic processes. It is not only an independent enzyme but also works with other lipid-metabolizing enzymes to form complexes. This involvement in the dynamic regulation of lipid utilization and storage influences cellular energy balance and metabolic flexibility. ACSL1 also associates with peroxisomes contributing to its functions in lipid metabolism and organelle integrity.

Pathways

ACSL1 holds a significant position in the fatty acid metabolism and PPAR signaling pathways. It interacts with enzymes like CPT1 important for transporting activated fatty acids into mitochondria for β-oxidation highlighting its integral role in energy harvesting from lipids. This participation in essential metabolic pathways demonstrates ACSL1's contribution to maintaining cellular energy and lipid balance.

ACSL1 links to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease. Overexpression or aberrant function of ACSL1 can disrupt normal lipid homeostasis contributing to abnormal lipid accumulation and metabolic dysregulation. The enzyme also associates with SREBP-1 a regulator of lipid synthesis indicating its involvement in lipid-related disorders. Researching ACSL1 helps understand its impact on lipid metabolism-related pathologies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially uses palmitoleate, oleate and linoleate (PubMed : 24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).
See full target information ACSL1
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 9:43 PubMed39738050

2024

Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte-myofibroblast transition in epidural fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Hua,Jinpeng Sun,Mohan Shi,Rui Mei,Zeyuan Song,Jun Liu,Mingshun Zhang

Cell death & disease 13:912 PubMed36309482

2022

Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Minghua Yang,Ke Liu,Pan Chen,Hongyi Zhu,Junjie Wang,Jun Huang

Stem cell research & therapy 12:330 PubMed34099046

2021

Rspo3 regulates the abnormal differentiation of small intestinal epithelial cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Han Yue,Xue-Guo Sun,Yue-Ping Jiang,Li Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com