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Rabbit Recombinant Monoclonal ACSL1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal ACSL1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR13498

Purity

Tissue culture supernatant

Specificity

This antibody shows very weak cross reactivity with ACSL5 and ACSL6.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

ACSL1 affects energy production and lipid storage by activating fatty acids making them substrates for metabolic processes. It is not only an independent enzyme but also works with other lipid-metabolizing enzymes to form complexes. This involvement in the dynamic regulation of lipid utilization and storage influences cellular energy balance and metabolic flexibility. ACSL1 also associates with peroxisomes contributing to its functions in lipid metabolism and organelle integrity.

Activity summary

The enzyme ACSL1 known as Acyl-CoA synthetase long-chain family member 1 plays an important role in lipid metabolism by converting free long-chain fatty acids into fatty acyl-CoA esters. This reaction is essential for the β-oxidation and synthesis of complex lipids. ACSL1 has an approximate mass of 78 kDa and is expressed in tissues with high metabolic activity including the liver heart and adipose tissue. The enzyme functions by regulating the cellular levels of fatty acids and their derivatives significantly impacting energy homeostasis.

Pathways

ACSL1 holds a significant position in the fatty acid metabolism and PPAR signaling pathways. It interacts with enzymes like CPT1 important for transporting activated fatty acids into mitochondria for β-oxidation highlighting its integral role in energy harvesting from lipids. This participation in essential metabolic pathways demonstrates ACSL1's contribution to maintaining cellular energy and lipid balance.

Associated diseases and disorders

ACSL1 links to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease. Overexpression or aberrant function of ACSL1 can disrupt normal lipid homeostasis contributing to abnormal lipid accumulation and metabolic dysregulation. The enzyme also associates with SREBP-1 a regulator of lipid synthesis indicating its involvement in lipid-related disorders. Researching ACSL1 helps understand its impact on lipid metabolism-related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACSL1 with ab178419 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419)

    All lanes: Western blot - Anti-ACSL1 antibody [EPR13498] (AB178419) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 78 kDa

  • Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419)

    ** Lanes 1-3:** Merged signal (red and green). Green - ab178419 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
    ab178419 Anti-ACSL1 antibody [EPR13498] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab178419 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ACSL1 antibody [EPR13498] (AB178419) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ACSL1 knockout HeLa cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (AB216773) at 1/10000 dilution

    Predicted band size: 78 kDa

    Observed band size: 78 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab178419).
    ** Lanes 1-3:** Merged signal (red and green). Green - ab178419 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
    ab178419 Anti-ACSL1 antibody [EPR13498] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab178419 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACSL1 with ab178419 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACSL1 with ab178419 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419), expandable thumbnail

    Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419)

    Blocking and diluting buffer: 5% NFDM/TBST.

    ab181602 was used as a loading control at 1/1000000 dilution.

    ab205606 was used as a loading control at 1/1000 dilution.

    This antibody shows very weak cross reactivity with ACSL5 and ACSL6.

    All lanes: Western blot - Anti-ACSL1 antibody [EPR13498] (AB178419) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector whole cell lysate at 10 µg

    Lane 2: 293T transfected with a human ACSL1 expression vector containing a DDDDK tag whole cell lysate at 10 µg

    Lane 3: 293T transfected with a human ACSL5 expression vector containing a DDDDK tag whole cell lysate

    Lane 4: 293T transfected with a human ACSL6 expression vector containing a DDDDK tag whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 75 kDa

    Exposure time: 20s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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