Anti-ACSL1 antibody [EPR13498] - BSA and Azide free

• WB

Unknown

Western blot - Anti-ACSL1 antibody [EPR13498] - BSA and Azide free (AB250044)

This data was developed using the same antibody clone in a different buffer formulation (ab178419). ** Lanes 1-3 : ** Merged signal (red and green). Green - ab178419 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa. ab178419 Anti-ACSL1 antibody [EPR13498] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab178419 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ACSL1 antibody [EPR13498] (<a href='/en-us/products/primary-antibodies/acsl1-antibody-epr13498-ab178419'>ab178419</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ACSL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ACSL1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-acsl1-knockout-hela-cell-line-ab265555'>ab265555</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

false

• WB

Supplier Data

Western blot - Anti-ACSL1 antibody [EPR13498] - BSA and Azide free (AB250044)

This data was developed using the same antibody clone in a different buffer formulation (ab178419).

Blocking and diluting buffer : 5% NFDM/TBST.

ab181602 was used as a loading control at 1/1000000 dilution.

ab205606 was used as a loading control at 1/1000 dilution.

This antibody shows very weak cross reactivity with ACSL5 and ACSL6.

All lanes:

Western blot - Anti-ACSL1 antibody [EPR13498] (<a href='/en-us/products/primary-antibodies/acsl1-antibody-epr13498-ab178419'>ab178419</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector whole cell lysate at 10 µg

Lane 2:

293T transfected with a human ACSL1 expression vector containing a DDDDK tag whole cell lysate at 10 µg

Lane 3:

293T transfected with a human ACSL5 expression vector containing a DDDDK tag whole cell lysate

Lane 4:

293T transfected with a human ACSL6 expression vector containing a DDDDK tag whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 75 kDa

false

Exposure time: 20s