AB308162

Anti-ACSL1 antibody [HL1530]

Recombinant
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Rabbit Recombinant Monoclonal ACSL1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL1.

View Alternative Names

FACL1, FACL2, LACS, LACS1, LACS2, ACSL1, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, Phytanate--CoA ligase, ACS1, LACS 1, LACS 2

4 Images

Supplier Data

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ACSL1 antibody [HL1530] (ab308162) at 1/2000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Lane 3:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ACSL1 antibody [HL1530] (ab308162) at 1/20000 dilution

All lanes:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 78 kDa

false

Supplier Data

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

All lanes:

Western blot - Anti-ACSL1 antibody [HL1530] (ab308162) at 1/20000 dilution

Lane 1:

Mouse liver tissue lysate at 50 µg

Lane 2:

Mouse spleen tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 78 kDa

false

Supplier Data

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ACSL1 antibody [HL1530] (ab308162) at 1/2000 dilution

All lanes:

NIH-3T3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 77 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1530

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ACSL1. The exact immunogen used to generate this antibody is proprietary information.

P33121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme ACSL1 known as Acyl-CoA synthetase long-chain family member 1 plays an important role in lipid metabolism by converting free long-chain fatty acids into fatty acyl-CoA esters. This reaction is essential for the β-oxidation and synthesis of complex lipids. ACSL1 has an approximate mass of 78 kDa and is expressed in tissues with high metabolic activity including the liver heart and adipose tissue. The enzyme functions by regulating the cellular levels of fatty acids and their derivatives significantly impacting energy homeostasis.

Biological function summary

ACSL1 affects energy production and lipid storage by activating fatty acids making them substrates for metabolic processes. It is not only an independent enzyme but also works with other lipid-metabolizing enzymes to form complexes. This involvement in the dynamic regulation of lipid utilization and storage influences cellular energy balance and metabolic flexibility. ACSL1 also associates with peroxisomes contributing to its functions in lipid metabolism and organelle integrity.

Pathways

ACSL1 holds a significant position in the fatty acid metabolism and PPAR signaling pathways. It interacts with enzymes like CPT1 important for transporting activated fatty acids into mitochondria for β-oxidation highlighting its integral role in energy harvesting from lipids. This participation in essential metabolic pathways demonstrates ACSL1's contribution to maintaining cellular energy and lipid balance.

ACSL1 links to metabolic disorders such as non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease. Overexpression or aberrant function of ACSL1 can disrupt normal lipid homeostasis contributing to abnormal lipid accumulation and metabolic dysregulation. The enzyme also associates with SREBP-1 a regulator of lipid synthesis indicating its involvement in lipid-related disorders. Researching ACSL1 helps understand its impact on lipid metabolism-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially uses palmitoleate, oleate and linoleate (PubMed : 24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

See full target information ACSL1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACSL1 antibody [HL1530]

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Western blot - Anti-ACSL1 antibody [HL1530] (AB308162)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise